Exposure to lipid-rich follicular fluid is associated with endoplasmic reticulum stress and impaired oocyte maturation in cumulus-oocyte complexes
Exposure to lipid-rich follicular fluid is associated with endoplasmic reticulum stress and impaired oocyte maturation in cumulus-oocyte complexes
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暴露于富含脂质的卵泡液与卵丘-卵母细胞复合物中的内质网应激和卵母细胞成熟受损有关
DOI：
10.1016/j.fertnstert.2012.02.034
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发表时间：
2012-06-01
影响因子：
6.7
通讯作者：
Robker, Rebecca L.
中科院分区：
文献类型：
--
作者：
Yang, Xing;Wu, Linda L.;Robker, Rebecca L.
目的：为了确定人类卵泡液的高脂质含量是否影响卵母细胞成熟。设计：小鼠卵母细胞作为人类卵母细胞的替代品暴露于不同脂质含量的卵泡液中，并进行结果监测。设置：私人不孕症诊所和大学实验室。患者：74名寻求辅助生殖的妇女和促性腺激素刺激的小鼠。干预措施：测定卵泡液中甘油三酯和游离脂肪酸的含量，以及在富脂或贫脂卵泡液中刺激小鼠卵丘-卵母细胞复合体（COCs）体外成熟。主要结果测量：卵母细胞脂质含量，内质网应激标记基因的表达，将暴露于富含脂质的卵泡液的小鼠COC中评估的卵母细胞成熟与暴露于贫脂质的卵泡液的复合物和体内成熟的复合物进行比较。卵泡液来自在私人不孕症诊所接受卵母细胞抽吸的已知体重指数的妇女，并测定卵泡液中的甘油三酯和游离脂肪酸;选择这些脂质水平最高和最低的卵泡液。暴露于富含脂质的卵泡液中的小鼠COCs在其成熟过程中，卵母细胞脂质含量增加，诱导内质网应激标志物，卵母细胞核成熟受损。结论：体重指数增加与卵巢卵泡液中甘油三酯和游离脂肪酸升高有关。在这种富含脂质的环境中成熟对卵母细胞是有害的。（Fertil Steril（R）2012;97：1438-43. (C)美国生殖医学会（American Society for Reproductive Medicine）
Objective: To determine whether the high lipid content of human follicular fluid influences oocyte maturation.Design: Mouse oocytes as substitutes for human oocytes were exposed to follicular fluids of differing lipid content with outcome monitoring.Setting: Private infertility clinic and university laboratory.Patient(s): Seventy-four women seeking assisted reproduction, and gonadotropin-stimulated mice.Intervention(s): Assay of follicular fluids for triglyceride and free fatty acids, and stimulation of mouse cumulus-oocyte complexes (COCs) to maturity in vitro in the presence of lipid-rich or lipid-poor follicular fluid.Main Outcome Measure(s): Oocyte lipid content, expression of endoplasmic reticulum stress marker genes, and oocyte maturation assessed in mouse COCs exposed to lipid-rich follicular fluid were compared with complexes exposed to lipid-poor follicular fluid and complexes matured in vivo.Result(s): Follicular fluids were obtained from women of known body mass index undergoing oocyte aspiration at a private infertility clinic, and the follicular fluids were assayed for triglyceride and free fatty acids; those with the highest and lowest levels of these lipids were selected. The mouse COCs exposed to lipid-rich follicular fluid during their maturation had increased oocyte lipid content, induction of endoplasmic reticulum stress markers, and impaired oocyte nuclear maturation.Conclusion(s): Increased body mass index is associated with elevated triglycerides and free fatty acids in ovarian follicular fluid. Maturation within this lipid-rich environment is detrimental to oocytes. (Fertil Steril (R) 2012;97:1438-43. (C) 2012 by American Society for Reproductive Medicine.)