A dopamine antagonist blocks vaginocervical stimulation-induced neuronal responses in the rat forebrain.
A dopamine antagonist blocks vaginocervical stimulation-induced neuronal responses in the rat forebrain.
复制标题
多巴胺拮抗剂可阻断阴道宫颈刺激引起的大鼠前脑神经元反应。
DOI：
10.1016/s0006-8993(01)03116-x
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Blaustein,JD
中科院分区：
文献类型：
--
作者：
Quysner,A;Blaustein,JD
在大鼠交配过程中，雄性通过性交向雌性提供阴道宫颈刺激（VCS）。手动提供的 VCS 模仿交配的许多方面，包括促进脊柱前凸、诱导性接受、缩短性接受期以及诱导每日两次催乳素激增（从而导致假妊娠）。 VCS 还诱导 Fos 的表达，Fos 是立即早期基因 c-fos 的蛋白质产物，已被用作对交配刺激做出反应的神经元的标记。由于 VCS 诱导前脑中多巴胺的释放，以及 DARPP-32（一种与多巴胺受体 D1 亚型激活相关的磷蛋白）的磷酸化，因此我们测试了 VCS 通过作用于 D1 类多巴胺受体来诱导 Fos 表达的假设。注射 SCH 23390（D1 类多巴胺受体拮抗剂）几乎消除了 VCS 诱导的 Fos 表达，而不影响 VCS 诱导 Fos 表达的所有脑区的 Fos 免疫反应性 (Fos-IR) 的组成水平。在后续实验中，第二种即刻早期蛋白 egr-1 的表达也被阻断，这表明这些结果并非 Fos 特有的。因此，结果与 VCS 诱导多巴胺释放、引起 D1 多巴胺受体激活，进而导致神经元反应的观点一致，如 Fos 和 egr-1 表达所示。
During mating in rats, the male provides vaginocervical stimulation (VCS) to the female via intromissions. VCS, provided manually, mimics many aspects of mating, including facilitation of lordosis, induction of sexual receptivity, abbreviation of the period of sexual receptivity, and induction of twice-daily prolactin surges, which result in pseudopregnancy. VCS also induces the expression of Fos, the protein product of the immediate early gene c-fos, which has been used as a marker for neurons that are responsive to mating stimuli. Because VCS induces the release of dopamine in the forebrain, as well as phosphorylation of DARPP-32, a phosphoprotein associated with activation of the D1subtype of dopamine receptor, we tested the hypothesis that VCS induces Fos expression by acting on the D1class of dopamine receptors. Injection of SCH 23390, an antagonist of the D1class of dopamine receptors, virtually eliminated VCS-induced Fos expression without affecting constitutive levels of Fos-Immunoreactivity (Fos-IR) in all brain areas in which VCS induced Fos expression. In a follow-up experiment, expression of a second immediate early protein, egr-1, was blocked as well, suggesting that these results are not specific to Fos. Therefore, the results are consistent with the idea that VCS induces dopamine release, causing activation of D1dopamine receptors, which in turn, results in neuronal response, as seen by both Fos and egr-1 expression.