Molecular or morphological species? Myxomycete diversity in a deciduous forest in northeastern Germany
Molecular or morphological species? Myxomycete diversity in a deciduous forest in northeastern Germany
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DOI：
10.1127/nova_hedwigia/2016/0326
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发表时间：
2017-01-01
期刊：
影响因子：
1
通讯作者：
Schnittler, Martin
中科院分区：
文献类型：
--
作者：
Feng, Yun;Schnittler, Martin
为了比较形态和分子多样性的木质黏菌，DNA样本中发现的所有标本的调查覆盖晚秋方面的粗木质残体上的黏菌friction进行了测序，使用部分序列的核小亚基核糖体RNA基因（SSU）作为条形码标记。从2012年10月初到12月初，对德国东北部Eldena古老森林中的欧洲山毛榉（Fagus sylvatica）的161根原木进行了调查，结果是530个收集物，代表14属的27种形态种（26种和1变种）。暗孢子类群的丰度（508个记录/17个类群/50个核糖体型）远远高于暗孢子类群（22个记录/10个类群/15个核糖体型）。根据形态种和核糖体型构建的积累曲线均收敛，根据Chao 2估计量，预计分类单元/核糖体型多样性的数字为32.6 +/- 4.9 / 90.0 +/- 11.1。基于部分SSU序列的亮孢子黏菌的系统发育揭示形态种在很大程度上是一致的系统发育群。大多数，但不是所有的强烈采样形态种包含多个核糖体不能区分的光学显微镜。这项研究表明，SSU序列可以作为黏菌的可靠条形码标记，但这些也揭示了一个显着的，但不是无限的，隐藏的多样性。
To compare morphological and molecular diversity for lignicolous myxomycetes, DNA samples of all specimens found within a survey covering the late-autumn aspect of myxomycete fructifications on coarse woody debris were sequenced, using partial sequences of the nuclear small subunit ribosomal RNA gene (SSU) as a barcode marker. A total of 161 logs, mostly from European beech (Fagus sylvatica) in the old-growth forest of Eldena in northeastern Germany, was surveyed from early October to early December 2012, resulting in 530 collections representing 27 morphospecies (26 species and one variety) from 14 genera. Bright-spored taxa were far more abundant (508 records/17 taxa/50 ribotypes) than dark-spored ones (22 records/10 taxa/15 ribotypes). Accumulation curves constructed from morphospecies and ribotypes both converged, resulting in figures of 32.6 +/- 4.9 / 90.0 +/- 11.1 for taxon / ribotype diversity to be expected according to the Chao2 estimator. A phylogeny based on partial SSU sequences for bright-spored myxomycetes revealed morphospecies to be largely consistent with phylogenetic groups. Most but not all intensely sampled morphospecies contain multiple ribotypes that cannot be differentiated by light microscopy. The study demonstrates that SSU sequences can function as reliable barcode markers for myxomycetes, but these also reveal a significant, yet not infinite, amount of hidden diversity.