Utilization of SABRE-Derived Hyperpolarization To Detect Low-Concentration Analytes via 1D and 2D NMR Methods
Utilization of SABRE-Derived Hyperpolarization To Detect Low-Concentration Analytes via 1D and 2D NMR Methods
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DOI：
10.1021/ja3051052
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发表时间：
2012-08-08
影响因子：
15
通讯作者：
Sleigh, Christopher J.
中科院分区：
文献类型：
--
作者：
Lloyd, Lyrelle S.;Adams, Ralph W.;Sleigh, Christopher J.
通过固有的不灵敏的NMR光谱方法对材料进行表征在化学中起着至关重要的作用。超极化越来越多地用于解决灵敏度限制问题。在这里，通过参考喹啉，我们说明了SABRE超极化技术，它使用仲氢作为极化源，能够快速完成一系列的NMR测量。这些包括C-13、C-13{H-1}和NOE数据的收集，以及更复杂的2D COSY、超快2D COSY和2D HMBC光谱。通过使用流动探针和外部样品制备池使观察成为可能，以在瞬变之间重新超滤底物，从而允许在几秒钟内进行重复测量。因此，建立了SABRE和2D NMR方法的组合用于低浓度分析物的快速表征的潜在益处。
The characterization of materials by the inherently insensitive method of NMR spectroscopy plays a vital role in chemistry. Increasingly, hyperpolarization is being used to address the sensitivity limitation. Here, by reference to quinoline, we illustrate that the SABRE hyperpolarization technique, which uses para-hydrogen as the source of polarization, enables the rapid completion of a range of NMR measurements. These include the collection of C-13, C-13{H-1}, and NOE data in addition to more complex 2D COSY, ultrafast 2D COSY and 2D HMBC spectra. The observations are made possible by the use of a flow probe and external sample preparation cell to re-hyperpolarize the substrate between transients, allowing repeat measurements to be made within seconds. The potential benefit of the combination of SABRE and 2D NMR methods for rapid characterization of low-concentration analytes is therefore established.