Inhibition of acid secretion from parietal cells by non-human-infecting Helicobacter species: a factor in colonization of gastric mucosa?
Inhibition of acid secretion from parietal cells by non-human-infecting Helicobacter species: a factor in colonization of gastric mucosa?
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DOI：
10.1128/iai.59.10.3694-3699.1991
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发表时间：
1991-10
影响因子：
3.1
通讯作者：
Madeline Vargas;A. Lee;James G. Fox;David R. Cave
中科院分区：
文献类型：
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作者：
Madeline Vargas;A. Lee;James G. Fox;David R. Cave
幽门螺杆菌已被证明会产生一种抑制顶叶细胞酸分泌的蛋白质。我们已经通过测量[14C]氨基吡啶对兔壁细胞的摄取来研究该特性的其他非人类感染的螺旋杆菌物种，作为对酸分泌的间接评估。显示出恒河杆菌和来自恒河猴的分离物可抑制酸分泌。紫脑细菌的分离株产生了可变的反应。整个细菌和无细胞超声层损害了[14C]氨基吡啶的摄取。我们还测试了其他细菌，包括大肠杆菌，普罗维亚氏菌，克莱伯斯ella oxytoca和弯曲杆菌空肠。作为整个生物体，这些对照细菌对酸的分泌几乎没有影响，但是超声的抑制作用明显，这部分是热不稳定的。幽门螺杆菌的原酶治疗破坏了其抑制作用。这些结果表明，大多数螺旋杆菌物种（但不是全部分离株）能够抑制兔顶细胞中的酸分泌。该特性可能是这些物种长期感染的一个因素。
Helicobacter pylori has been shown to produce a protein that inhibits acid secretion from parietal cells. We have examined other non-human-infecting Helicobacter species for this property by measuring the uptake of [14C]aminopyrine into rabbit parietal cells as an indirect assessment of acid secretion. Helicobacter felis and an isolate from a rhesus monkey were shown to inhibit acid secretion. Isolates of Helicobacter mustelae gave variable responses. Whole bacteria and cell-free sonicates impaired the uptake of [14C]aminopyrine. We also tested other bacteria, including Escherichia coli, Proteus vulgaris, Klebsiella oxytoca, and Campylobacter jejuni. As whole organisms, these control bacteria had little effect on acid secretion, but sonicates caused pronounced inhibition that was partially heat labile. Pronase treatment of H. pylori destroyed its inhibitory effect. These results suggest that most Helicobacter species, but not all isolates, are able to inhibit acid secretion from rabbit parietal cells. This property may be a factor in the establishment of long-term infection by these species.