Centrally and peripherally administered ghrelin potently inhibits water intake in rats
Centrally and peripherally administered ghrelin potently inhibits water intake in rats
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DOI：
10.1210/en.2006-0993
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发表时间：
2007-04-01
期刊：
影响因子：
4.8
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Hashimoto, Hirofumi;Fujihara, Hiroaki;Ueta, Yoichi
Ghrelin通过其对大脑的作用而被认为是一种有效的食欲激素。本研究观察了脑室内（icv）和静脉注射ghrelin对大鼠饮水量、摄食量和尿量的影响脑室内注射ghrelin（0.1、1和10 nmol/rat）以剂量相关的方式显著抑制水剥夺后发生的水摄入，尽管食物摄入受到激素的刺激。抗渴作用与食欲作用一样有效。同样，水的摄入量被抑制，而食物的摄入量刺激剂量依赖性静脉注射后的生长激素释放肽（0.1，1，和10 nmol/kg）。抑制饮酒是可比的，甚至更有效的比，心钠素（ANP），一个既定的antidipsogenic激素，icv时，虽然antidipsogenic效果持续时间更长。ANP对摄食量无影响。侧脑室注射ghrelin后尿量呈剂量相关性减少，但ANP无此作用。静脉注射胃饥饿素对尿量没有影响。由于饮酒通常发生在进食时，因此撤去食物以消除餐时饮酒。然后，生长激素释放肽的抗渴作用变得比ANP更有效，并且持续时间比食物可用时更长。免疫组化结果显示，侧脑室和静脉注射Ghrelin后，大鼠孤束核和最后区Fos表达增加。目前的研究令人信服地表明，生长激素释放肽是一种有效的抗disogenic肽在大鼠。
Ghrelin is known as a potent orexigenic hormone through its action on the brain. In this study, we examined the effects of intracerebroventricular (icv) and iv injection of ghrelin on water intake, food intake, and urine volume in rats deprived of water for 24 h. Water intake that occurred after water deprivation was significantly inhibited by icv injection of ghrelin (0.1, 1, and 10 nmol/rat) in a dose-related manner, although food intake was stimulated by the hormone. The antidipsogenic effect was as potent as the orexigenic effect. Similarly, water intake was inhibited, whereas food intake was stimulated dose dependently after iv injection of ghrelin (0.1, 1, and 10 nmol/kg). The inhibition of drinking was comparable with, or even more potent than, atrial natriuretic peptide (ANP), an established antidipsogenic hormone, when administered icv, although the antidipsogenic effect lasted longer. ANP had no effect on food intake. Urine volume decreased dose relatedly after icv injection of ghrelin but not by ANP. Intravenous injection of ghrelin had no effect on urine volume. Because drinking usually occurs with feeding, food was withdrawn to remove the prandial drinking. Then the antidipsogenic effect of ghrelin became more potent than that of ANP and continued longer than when food was available. Expression of Fos was increased in the area postrema and the nucleus of the tractus solitarius by using immunohistochemistry after icv and iv injection of ghrelin. The present study convincingly showed that ghrelin is a potent antidisogenic peptide in rats.