The forkhead box F1 transcription factor is expressed in brain and head mesenchyme during mouse embryonic development
The forkhead box F1 transcription factor is expressed in brain and head mesenchyme during mouse embryonic development
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DOI：
10.1016/s1567-133x(03)00010-3
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发表时间：
2003-05-01
影响因子：
1.2
通讯作者：
Costa, RH
中科院分区：
文献类型：
--
作者：
Kalinichenko, VV;Gusarova, GA;Costa, RH
叉头盒F1(FOXF1)转录因子在横隔间充质和内脏(内脏)中胚层中表达，可分化出肝、胆、肺、胃、肠等肠源性器官的间充质细胞。Foxf1-/-胚胎在子宫内死亡，Foxf1基因单倍体不足导致肺、胆、食道和气管的各种发育异常，并导致肝和肺修复缺陷。在这项研究中，我们利用含有β-半乳糖苷酶(β-Gal)基因的Foxf1+/-小鼠胚胎敲入Foxf1基因座，以检测Foxf1是否在发育中的大脑和头部区域表达。除已报道的Foxf1表达模式外，在发育中的脑下垂体、眼、胰腺、心脏、脊索、生殖器结节、Meckel‘s软骨以及鼻中隔和椎体的软骨原基的间质中也发现了β-Gal染色。在成年Foxf1+/-小鼠中，β-Gal染色见于脑垂体腺前部和后部、小脑和大脑皮质的星形胶质细胞、眼的晶状体和视网膜、气管软骨、甲状旁腺、胸腺皮质层、脾被膜、冠状动脉和胰腺血管。(C)2003 Elsevier Science B.V.保留所有权利。
The forkhead box f1 (Foxf1) transcription factor is expressed in septum transversum mesenchyme and splanchnic (visceral) mesoderm, which give rise to mesenchymal cells of gut-derived organs such as liver, gall bladder, lung, stomach, and intestine. Foxf1 -/- embryos die in utero and haploinsufficiency of the Foxf1 gene resulted in a variety of developmental abnormalities of the lung, gall bladder, esophagus, and trachea and caused defects in liver and lung repair. In this study, we used Foxf1 +/- mouse embryos containing the beta-Galactosidase (beta-Gal) gene knocked into the Foxf1 locus to examine whether Foxf1 is expressed in the developing brain and head region. In addition to previously reported Foxf1 expression patterns, beta-Gal staining was also found in the mesenchyme of developing pituitary gland, eye, pancreas, heart, notochord, genital tubercle, Meckel's cartilage, and cartilage primordia of nasal septum and vertebral bodies. In adult Foxf1 +/- mice, beta-Gal staining was detected in anterior and posterior pituitary gland, astrocytes of the cerebellum and cerebral cortex, lens and retina of the eye, tracheal cartilage, parathyroid, cortical layer of thymus, capsule of the spleen, coronary arteries, and pancreatic blood vessels. (C) 2003 Elsevier Science B.V. All rights reserved.