Contribution of <i>sox9b</i> to pigment cell formation in medaka fish
Contribution of <i>sox9b</i> to pigment cell formation in medaka fish
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<i>sox9b</i> 对青鳉鱼色素细胞形成的贡献
DOI：
10.1111/dgd.12760
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hashimoto Hisashi
中科院分区：
文献类型：
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作者：
Tsunogai Yuri;Miyadai Motohiro;Nagao Yusuke;Sugiwaka Keisuke;Kelsh Robert N.;Hibi Masahiko;Hashimoto Hisashi
SoxE类转录因子Sox10和Sox9是神经脊细胞发育的关键调控因子。Sox10规定了色素细胞、神经胶质细胞和神经元的谱系，而Sox9据报道与头部的骨骼形成谱系密切相关，但它参与色素细胞的形成尚未进行遗传学研究。因此，硬骨鱼中的这些基因以及它们的同源基因是否或如何被亚功能化还没有完全弄清楚。我们之前已经用青黛鱼水稻表明，色素细胞的形成受到Sox10a损失的严重影响，但不受Sox10b损失的影响。在这里，我们的目的是确定Sox9是否参与了色素细胞谱系的指定。毒素9纯合子突变不影响色素细胞的形成，尽管在幼虫早期阶段是致命的。利用sox10a、sox10b和sox9突变，建立了ox9band sox10基因的复合突变体，并对其色素细胞表型进行了分析。Sox9band sox10a的同时缺失导致幼体完全缺乏黑色素和黄色素，虹膜团形成严重缺陷，就像先前在sox10a−/−；sox10b−/−双突变体中所显示的那样，这表明Sox9b和Sox10b在色素细胞发育中与Sox10a存在冗余功能。值得注意的是，存在insox9b−/−；sox10a−/−andsox10a−/−；sox10b−/−double突变体，但它们的数量在ox9b−/−；sox10a−/−突变体中显著减少。这些发现突显了Sox9b参与了色素细胞的形成，并且在白细胞体的发育中比Sox10b起到了更关键的作用。
SoxE‐type transcription factors, Sox10 and Sox9, are key regulators of the development of neural crest cells. Sox10 specifies pigment cell, glial, and neuronal lineages, whereas Sox9 is reportedly closely associated with skeletogenic lineages in the head, but its involvement in pigment cell formation has not been investigated genetically. Thus, it is not fully understood whether or how distinctly these genes as well as their paralogs in teleosts are subfunctionalized. We have previously shown using the medaka fishOryzias latipesthat pigment cell formation is severely affected by the loss ofsox10a, yet unaffected by the loss ofsox10b. Here we aimed to determine whether Sox9 is involved in the specification of pigment cell lineage. Thesox9bhomozygous mutation did not affect pigment cell formation, despite lethality at the early larval stages. By usingsox10a,sox10b, andsox9bmutations, compound mutants were established for thesox9bandsox10genes and pigment cell phenotypes were analyzed. Simultaneous loss ofsox9bandsox10aresulted in the complete absence of melanophores and xanthophores from hatchlings and severely defective iridophore formation, as has been previously shown forsox10a−/−;sox10b−/−double mutants, indicating that Sox9b as well as Sox10b functions redundantly with Sox10a in pigment cell development. Notably, leucophores were present insox9b−/−;sox10a−/−andsox10a−/−;sox10b−/−double mutants, but their numbers were significantly reduced in thesox9b−/−;sox10a−/−mutants. These findings highlight that Sox9b is involved in pigment cell formation, and plays a more critical role in leucophore development than Sox10b.