Insights into Sex Chromosome Evolution and Aging from the Genome of a Short-Lived Fish
Insights into Sex Chromosome Evolution and Aging from the Genome of a Short-Lived Fish
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DOI：
10.1016/j.cell.2015.10.071
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发表时间：
2015-12-06
期刊：
影响因子：
64.5
通讯作者：
Platzer, Matthias
中科院分区：
文献类型：
--
作者：
Reichwald, Kathrin;Petzold, Andreas;Platzer, Matthias
鳉鱼 Nothobranchius Furzeri 是可在实验室培育的寿命最短的脊椎动物。它的快速生长、早期性成熟、快速衰老和胚胎发育停滞（滞育）使其成为生物医学研究中有吸引力的模型生物。在这里，我们报告了其基因组序列草图，使我们能够揭示一种种内 Y 染色体多态性，它实时代表性染色体形成的不同阶段，显示出早期哺乳动物 XY 进化“正在发生”的特征。我们的数据表明，编码 TGF-β 家族生长因子的 gdf6Y 是毛茸茸的主要性别决定基因。此外，我们观察了衰老相关基因的基因组聚类，鉴定了正选择下的基因，并揭示了滞育和衰老之间基因表达谱的显着相似性，特别是控制细胞周期和翻译的基因。带注释的基因组序列作为在线资源提供（http://www.nothobranchius.info/NFINgb）。
The killifish Nothobranchius furzeri is the shortest-lived vertebrate that can be bred in the laboratory. Its rapid growth, early sexual maturation, fast aging, and arrested embryonic development (diapause) make it an attractive model organism in biomedical research. Here, we report a draft sequence of its genome that allowed us to uncover an intra-species Y chromosome polymorphism representing-in real time-different stages of sex chromosome formation that display features of early mammalian XY evolution "in action." Our data suggest that gdf6Y, encoding a TGF-beta family growth factor, is the master sex-determining gene in N. furzeri. Moreover, we observed genomic clustering of aging-related genes, identified genes under positive selection, and revealed significant similarities of gene expression profiles between diapause and aging, particularly for genes controlling cell cycle and translation. The annotated genome sequence is provided as an online resource (http://www.nothobranchius.info/NFINgb).