Metabolism of dynorphins by peptidases of pulmonary artery endothelial cells.
Metabolism of dynorphins by peptidases of pulmonary artery endothelial cells.
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肺动脉内皮细胞肽酶对强啡肽的代谢。
DOI：
10.1080/10623320210711
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Prokai,Laszlo
中科院分区：
文献类型：
--
作者：
Zharikova,Alevtina;Zharikov,Sergey;Block,EdwardR;Prokai,Laszlo
Degradation of several dynorphins by peptidases expressed in cultured porcine pulmonary artery endothelial cells was studied by incubation of the peptide in cell suspensions followed by electrospray ionization and tandem mass spectrometric analyses. Under the in vitro conditions applied, only the metabolism of dynorphin A 1-8 occurred in a significant extent. Studies involving specific peptidase inhibitors indicated that mainly bestatin-sensitive aminopeptidases, thiorphan-sensitive endopeptidases, and cFPAAF-pAB-sensitive endopeptidases expressed by the endothelial cells were involved in the process that converted dynorphin A 1-8 to dynorphin A 2-8 , dynorphin A 1-6 , and leucine enkephalin (dynorphin A 1-5 ), respectively. These peptidases may form a metabolic barrier for the cellular penetration of intact dynorphin A 1-8 and/or control effects of the circulating peptide on endothelial opioid receptors of the cells.
