Human antibody response to a pneumococcal vaccine in SCID‐PBL‐hu mice and simultaneously vaccinated human cell donors
Human antibody response to a pneumococcal vaccine in SCID‐PBL‐hu mice and simultaneously vaccinated human cell donors
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SCID-PBL-hu 小鼠和同时怀孕的人类细胞供体对肺炎球菌疫苗的人类抗体反应
DOI：
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发表时间：
1996
影响因子：
4.6
通讯作者：
M. Løvik
中科院分区：
文献类型：
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作者：
I. Aaberge;T. E. Steinsvik;E. Groeng;R. Leikvold;M. Løvik
将来自9个健康人供体的人外周血淋巴细胞（PBL）腹膜内移植到重度联合免疫缺陷（SCID）小鼠（SCID-PBL-hu小鼠）。没有一个捐献者曾经接种过肺炎球菌疫苗。移植后10天，111只移植小鼠中的62只和9只供体中的6只接种了23价肺炎球菌多糖疫苗。对于每个供体，在91.7 - 100%的SCID-PBL-hu小鼠中检测到人IgG，而在16.7 - 100%的接种SCID-PBL-hu小鼠中证明了特异性人IgG抗肺炎球菌抗体。大多数移植了来自同一供体的细胞的小鼠在动力学和抗体水平方面显示出相似的抗体应答模式。仅在接受移植时抗肺炎球菌抗体水平相对较高的供体细胞或接种后抗体水平大幅增加的供体细胞的小鼠中获得显著的抗体应答。SCID-PBL-hu小鼠的免疫应答并不总是反映相应供体产生抗肺炎球菌抗体的能力。抗肺炎球菌抗体应答的供体依赖性对于SCID-PBL-hu模型的使用具有重要的实际意义。捐赠者不应该随机选择。通过选择其细胞已被发现导致成功植入的供体，可以获得功能性SCID-PBL-hu小鼠，用于在体内实验模型中研究人免疫应答和功能。
Severe combined immunodeficient (SCID) mice were transplanted intraperitoneally with human peripheral blood lymphocytes (PBL) from nine healthy human donors (SCID‐PBL‐hu mice). None of the donors had ever received pneumococcal vaccine. Ten days after transplantation, 62 out of 111 transplanted mice and six of the nine donors were vaccinated with a 23‐valent pneumococcal polysaccharide vaccine. For each donor, human IgG was detected in 91.7–100% of the SCID‐PBL‐hu mice, whereas specific human IgG antipneumococcal antibodies were demonstrated in 16.7–100% of the vaccinated SCID‐PBL‐hu mice. Most of the mice transplanted with cells from the same donor showed similar antibody response patterns in terms of kinetics and antibody levels. A significant antibody response was only obtained in mice that received cells from donors with relatively high antipneumococcal antibody levels at the time of transplantation, or donors that showed a substantial increase in antibody levels after vaccination. The immune response in the SCID‐PBL‐hu mice did not always reflect the ability of the respective donor to produce antipneumococcal antibodies. The donor dependency of the antipneumococcal antibody response has great practical importance for the use of the SCID‐PBL‐hu model. Donors should not be chosen randomly. By selecting donors whose cells have been found to result in successful engraftment, functional SCID‐PBL‐hu mice can be obtained for the study of human immune responses and function in an in vivo experimental model.
通过独特型疫苗接种在 hu-PBL SCID 小鼠中诱导保护性人多糖特异性抗体反应。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Reason,DC;Kitamura,MY;Lucas,AH
通讯作者：
Lucas,AH