Comparative transcriptomics provide new insights into the mechanisms by which foliar silicon alleviates the effects of cadmium exposure in rice
Comparative transcriptomics provide new insights into the mechanisms by which foliar silicon alleviates the effects of cadmium exposure in rice
复制标题
比较转录组学为叶面硅减轻水稻镉暴露影响的机制提供了新的见解
DOI：
10.1016/j.jes.2021.07.030
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发表时间：
2022-05-01
影响因子：
6.9
通讯作者：
Yi, Jicai
中科院分区：
文献类型：
--
作者：
Sun, Chongjun;Liang, Xiaoyu;Yi, Jicai
硅（Si）已被证明可以缓解水稻镉胁迫。本文研究了籼稻黄花占叶面施硅的效益。结果表明，叶面施硅仅在灌浆期增加了镉暴露水稻的干重，减少了镉的转运，说明灌浆期是叶面施硅减少镉积累的关键时期。我们还调查了旗叶（FLs），小穗（SPs），和节（NIs）的镉暴露HHZ叶面施硅后灌浆期的转录组。重要的是，与Si介导的Cd胁迫缓解相关的基因表达谱具有组织特异性，而在Cd胁迫下水稻组织中Si介导的途径是共享的。此外，Si处理后，FL和SP中ATP结合盒（ABC）转运蛋白表达上调，而FL和NI中二价阳离子转运蛋白表达下调，这可能有助于降低Cd积累。与必需营养物质转运蛋白、碳水化合物和次生代谢产物合成以及细胞色素氧化酶活性相关的基因在Cd暴露的FL和SP中大多被上调，这可能有助于缓解Cd暴露下的氧化胁迫，促进植物生长。感兴趣的是，负责信号转导的基因在FL中受到负调控，而在SP中受到正调控。我们的研究结果提供了转录组证据表明，叶片硅在缓解镉暴露的影响在水稻中起着积极的作用。特别是，叶面硅可以改变与运输，生物合成和代谢，氧化还原相关的基因的表达模式。(c)2021中国科学院生态环境科学研究中心由Elsevier B. V.发布。这是一个CC BY-NC-ND许可证下的开放获取文章（http://creativecommons.org/licenses/by-nc-nd/4.0/）
Silicon (Si) has been shown to alleviate Cd stress in rice. Here, we investigated the beneficial effects of foliar Si in an indica rice Huanghuazhan (HHZ). Our results showed that foliar Si in-creases the dry weight and decreases Cd translocation in Cd-exposed rice at the grain-filling stage only, implying that the filling stage is critical for foliar Si to reduce Cd accumulation. We also investigated the transcriptomics in flag leaves (FLs), spikelets (SPs), and node Is (NIs) of Cd-exposed HHZ after foliar Si application at the filling stage. Importantly, the gene expression profiles associated with the Si-mediated alleviation of Cd stress were tissue spe-cific, while shared pathways were mediated by Si in Cd-exposed rice tissues. Furthermore, after the Si treatment of Cd-exposed rice, the ATP-binding cassette (ABC)-transporters were mostly upregulated in FL and SP, while the bivalent cation transporters were mostly down-regulated in FL and NI, possibly helping to reduce Cd accumulation. The genes associated with essential nutrient transporters, carbohydrate and secondary metabolite biosynthesis, and cytochrome oxidase activity were mostly upregulated in Cd-exposed FL and SP, which may help to alleviate oxidative stress and improve plant growth under Cd exposure. Inter-estingly, genes responsible for signal transduction were negatively regulated in FL, but pos-itively regulated in SP, by foliar Si. Our results provide transcriptomic evidence that foliar Si plays an active role in alleviating the effects of Cd exposure in rice. In particular, foliar Si may alter the expression pattern of genes associated with transport, biosynthesis and metabolism, and oxidation reduction. (c) 2021 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )