CLOSE LINKAGE OF GENES SER-C (FOR PHOSPHOHYDROXY PYRUVATE TRANSAMINASE) AND SER-S (FOR SERYL-TRANSFER RIBONUCLEIC-ACID SYNTHETASE) IN ESCHERICHIA-COLI K-12
CLOSE LINKAGE OF GENES SER-C (FOR PHOSPHOHYDROXY PYRUVATE TRANSAMINASE) AND SER-S (FOR SERYL-TRANSFER RIBONUCLEIC-ACID SYNTHETASE) IN ESCHERICHIA-COLI K-12
复制标题
DOI：
10.1128/jb.113.3.1091-1095.1973
复制
发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
KONIGSBERG, WH
中科院分区：
文献类型：
--
作者：
CLARKE, SJ;LOW, B;KONIGSBERG, WH
亚硝基胍诱变后分离出的大肠杆菌 K28 被发现丝氨酸营养缺陷。由于产生改变的丝氨酰转移核糖核酸 (tRNA) 合成酶（EC 6.1.1.11，l-丝氨酸：tRNA 连接酶 [AMP]），它的生长对温度也敏感。营养缺陷型是由磷酸羟基丙酮酸转氨酶（serC）结构基因突变引起的，该基因与丝氨酰-tRNA合成酶（serS）结构基因不同，但密切相关。我们得出结论，相关基因位于 ordergal-serS-serC-aroA 中。
Escherichia colistrain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11,l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the ordergal-serS-serC-aroA.