Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods
Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods
复制标题
基于金纳米棒受控碘介导蚀刻的酸性磷酸酶智能手机比色测定
DOI：
10.1007/s00216-020-02954-5
复制
发表时间：
2020
影响因子：
4.3
通讯作者：
Fang-Ying Wu
中科院分区：
文献类型：
--
作者：
Bo-Wen Liu;Pengcheng Huang;Fang-Ying Wu
A simple but efficient colorimetric assay was developed for the detection and quantification of acid phosphatase (ACP) using a smartphone. This strategy is based on target-controlled iodine-mediated etching of gold nanorods (AuNRs). Due to effective hydrolysis of the substrate pyrophosphate (PPi) by ACP, chelated Cu2+with PPi was released, which promoted the redox reaction with an iodide ion (I−), leading to the formation of I3−. As the etching agent of AuNRs, I3−caused a blueshift of the localized surface plasmon resonance peak and, more importantly, an observable color change. The vivid colors were recorded with a smartphone camera and directly analyzed using an image-processing app. On the basis of the direct correlation between ACP concentration and the etching degree of AuNRs as well as color change, this smartphone nanocolorimetry technique showed a good linear response toward ACP over the range of 0–15.0 U/L, with a detection limit of 0.97 U/L. Using the standard addition method, the practical applicability of the proposed smartphone-based assay was successfully demonstrated by determining ACP in human serum samples, with results consistent with those obtained by UV-Vis spectrophotometry.
