Molecular cloning, characterization, and expression of a cDNA encoding the "80- to 87-kDa" myristoylated alanine-rich C kinase substrate: a major cellular substrate for protein kinase C.
Molecular cloning, characterization, and expression of a cDNA encoding the "80- to 87-kDa" myristoylated alanine-rich C kinase substrate: a major cellular substrate for protein kinase C.
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DOI：
10.1073/pnas.86.11.4012
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发表时间：
1989-06
影响因子：
11.1
通讯作者：
D. Stumpo;Jonathan M. Graff;Katherine A. Albert;Paul Greengard;P. J. Blackshear
中科院分区：
文献类型：
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作者：
D. Stumpo;Jonathan M. Graff;Katherine A. Albert;Paul Greengard;P. J. Blackshear
我们分离并测序了编码牛“80- 87-kDa”蛋白的cDNA克隆，该蛋白是蛋白激酶C的主要细胞底物。一个1005个碱基对的开放阅读框架预测了一个335个氨基酸的蛋白质（Mr，31，949）。尽管有这种预测的大小，但在缺乏该蛋白的细胞中表达cDNA后，该蛋白在SDS/聚丙烯酰胺凝胶上迁移，表观分子量为80- 87，000。它是高度富含丙氨酸（28.4摩尔%），含有一个氨基末端豆蔻酰化的共识序列，并包括一个25个残基的碱性结构域含有已知的蛋白激酶C磷酸化位点。两种mRNA种类（2.6和4.4酶）在脑、脊髓、脾和肺中表达最高，与免疫反应蛋白的分布平行。基因组印迹分析表明，一个单一的基因编码这种mRNA的可能性。我们建议名称豆蔻酰丙氨酸丰富的C激酶底物（MARCKS）的这种蛋白质。
We isolated and sequenced a cDNA clone encoding the bovine "80- to 87-kDa" protein, a major cellular substrate for protein kinase C. An open reading frame of 1005 base pairs predicted a protein of 335 amino acids (Mr, 31,949). Despite this predicted size, the protein migrated on SDS/polyacrylamide gels with an apparent molecular weight of 80-87,000 after expression of the cDNA in cells lacking the protein. It was highly enriched in alanine (28.4 mol %), contained an amino-terminal myristoylation consensus sequence, and included a 25-residue basic domain containing the known protein kinase C phosphorylation sites. Two mRNA species (2.6 and 4.4 kilobases) were most highly expressed in brain, spinal cord, spleen, and lung, in parallel with the distribution of immuno-reactive protein. Genomic blot analysis indicated the likelihood of a single gene coding for this mRNA. We propose the name myristoylated alanine-rich C kinase substrate (MARCKS) for this protein.