Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription-dependent mobility
Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription-dependent mobility
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DOI：
10.1091/mbc.e03-10-0743
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发表时间：
2004-04-01
影响因子：
3.3
通讯作者：
Powers, MA
中科院分区：
文献类型：
--
作者：
Griffis, ER;Craige, B;Powers, MA
尽管核孔复合物在相间期间的明显的整体结构稳定性，至少有两个核孔蛋白已被证明动态地移动和关闭的孔。目前尚不清楚核孔蛋白的流动性对核运输过程的贡献，以及这种流动性是如何调节的。以前，我们表明Nup98以转录依赖的方式与NPC以及核内的机构动态相互作用。我们已经扩展了我们的动力学研究，包括Nup153，另一个移动的核孔蛋白参与RNA输出。在这两种情况下，我们发现，虽然只有一个域是必不可少的NPC定位，其他地区的蛋白质显着影响与孔协会的稳定性。有趣的是，像Nup98一样，当Pol I和Pol II的转录被阻断时，Nup153在孔上和孔外的交换被抑制。我们已经绘制了Nup98和Nup153迁移率与转录之间的联系所需的区域，并发现这些要求取决于哪些聚合酶被抑制。我们的数据支持一个模型，即RNA的转录耦合到核孔蛋白的流动性，也许最终连接到一个周期的重塑在核孔篮的RNA的运输。
Despite the apparent overall structural stability of the nuclear pore complex during interphase, at least two nucleoporins have been shown to move dynamically on and off the pore. It is not yet certain what contribution nucleoporin mobility makes to the process of nuclear transport or how such mobility is regulated. Previously, we showed that Nup98 dynamically interacts with the NPC as well as bodies within the nucleus in a transcription-dependent manner. We have extended our studies of dynamics to include Nup153, another mobile nucleoporin implicated in RNA export. In both cases, we found that although only one domain is essential for NPC localization, other regions of the protein significantly affect the stability of association with the pore. Interestingly, like Nup98, the exchange of Nup153 on and off the pore is inhibited when transcription by Pol I and Pol II is blocked. We have mapped the regions required to link Nup98 and Nup153 mobility to transcription and found that the requirements differ depending on which polymerases are inhibited. Our data support a model whereby transcription of RNA is coupled to nucleoporin mobility, perhaps ultimately linking transport of RNAs to a cycle of remodeling at the nuclear pore basket.