Remodeling nanoDESI Platform with Ion Mobility Spectrometry to Expand Protein Coverage in Cancerous Tissue
Remodeling nanoDESI Platform with Ion Mobility Spectrometry to Expand Protein Coverage in Cancerous Tissue
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DOI：
10.1021/jasms.0c00354
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发表时间：
2021-01-28
影响因子：
3.2
通讯作者：
Hsu, Cheng-Chih
中科院分区：
文献类型：
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作者：
Chen, Chih-Lin;Kuo, Ting-Hao;Hsu, Cheng-Chih
纳米喷雾解吸电喷雾质谱法是一种能够在组织切片中定位蛋白质的环境电离技术。然而，生物样品中的高丰度分子或等压干扰阻碍了其在探测低丰度蛋白质方面的广泛应用。为了解决这一挑战，本文展示了一个集成模块，该模块将气动辅助纳米喷雾解吸电喷雾质谱与高场不对称离子迁移谱相结合。使用该模块分析小鼠脑切片，蛋白质覆盖率显著增加。这种改进允许在5 μ m的空间分辨率下绘制组织切片中的低丰度蛋白质，通过计算辅助融合光学显微镜图像。此外，该模块已成功应用于基于增强蛋白谱的皮肤组织黑色素瘤特征。结果表明，这种整合模块将有可能应用于发现癌症中的新蛋白质。
Nanospray desorption electrospray ionization mass spectrometry is an ambient ionization technique that is capable of mapping proteins in tissue sections. However, high-abundant molecules or isobaric interference in biological samples hampers its broad applications in probing low-abundant proteins. To address this challenge, herein we demonstrated an integrated module that coupled pneumatic-assisted nanospray desorption electrospray ionization mass spectrometry with high-field asymmetric ion mobility spectrometry. Using this module to analyze mouse brain sections, the protein coverage was significantly increased. This improvement allowed the mapping of low-abundant proteins in tissue sections with a 5 mu m spatial resolution enabled by computationally assisted fusion with optical microscopic images. Moreover, the module was successfully applied to characterize melanoma in skin tissues based on the enhanced protein profiles. The results suggested that this integrating module will be potentially applied to discover novel proteins in cancers.