Improved in vitro angiogenic behavior of human umbilical vein endothelial cells with oxidized polydopamine coating
Improved in vitro angiogenic behavior of human umbilical vein endothelial cells with oxidized polydopamine coating
复制标题
DOI：
10.1016/j.colsurfb.2020.111176
复制
发表时间：
2020-10-01
影响因子：
5.8
通讯作者：
Liu, Xuanyong
中科院分区：
文献类型：
--
作者：
Cheng, Shi;Wang, Donghui;Liu, Xuanyong
贻贝衍生的聚多巴胺（PDA）涂层因其上级的成骨性能而引起了人们的广泛关注。此外，最近的研究表明，血管形成是骨再生的关键。因此，在本研究中，不同氧化程度的聚多巴胺涂层的潜在能力进行了系统的研究，在体外提高人脐静脉内皮细胞（HUVECs）的血管生成行为。首先将PDA涂覆在钛（PDA-1#）上，然后分别通过在150 ℃（PDA-2#）和300 ℃（PDA-3#）下的热处理进行氧化。X射线光电子能谱（XPS）结果表明，氧化后PDA表面的酚羟基（C-OH）和伯氨基（-NH 2）减少，醌基（C=O）增加。体外细胞培养实验表明，PDA-2#样品最有利于HUVECs的粘附、迁移和增殖。PDA-2#培养的HUVECs具有最好的管形成、CD 31表达、血管内皮生长因子（VEGF）分泌以及血管生成相关基因表达能力。我们的研究表明，适度氧化的PDA涂层与平衡的醌和氨基具有良好的潜力，以促进骨血管化，因此是有希望的临床应用在骨科植入物。
Mussel inspired polydopamine (PDA) coatings have attracted a great deal of attention for their superior osteogenic property. Furthermore, recent investigations have demonstrated that vessel formation is crucial to bone regeneration. Hence, in the present study, the potential ability of polydopamine coatings with different oxidation degrees were systematically investigated in vitro to improve the angiogenic behavior of human umbilical vein endothelial cells (HUVECs). PDA was first coated on titanium (PDA-1#), and then oxidized by thermal treatment at 150 (PDA-2#) and 300 degrees C (PDA-3#), respectively. X-ray photoelectron spectroscopy (XPS) results revealed that phenolic hydroxyl (C-OH) and primary amino group (-NH2) on PDA coatings deceased after oxidation, while quinone (C=O) increased. In vitro cell culture experiments suggested that PDA-2# sample was most beneficial for the adhesion, migration, and proliferation of HUVECs. Furthermore, HUVECs cultured on PDA-2# sample also exhibited best tube formation, CD31 expression, vascular endothelial growth factor (VEGF) secretion, as well as angiogenic-associated gene expression abilities. Our study suggests that moderate oxidation of PDA coating with balanced quinone and amino group has excellent potential to enhance bone vascularization, and is thus promising for clinical application in orthopedic implants.