Physiological Properties and Genome Structure of the Hyperthermophilic Filamentous Phage φOH3 Which Infects Thermus thermophilus HB8.
Physiological Properties and Genome Structure of the Hyperthermophilic Filamentous Phage φOH3 Which Infects Thermus thermophilus HB8.
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DOI：
10.3389/fmicb.2016.00050
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发表时间：
2016
影响因子：
5.2
通讯作者：
Doi K
中科院分区：
文献类型：
--
作者：
Nagayoshi Y;Kumagae K;Mori K;Tashiro K;Nakamura A;Fujino Y;Hiromasa Y;Iwamoto T;Kuhara S;Ohshima T;Doi K
从日本奥巴马温泉沉积物中分离到一株噬菌体φ OH 3，其宿主为嗜热栖热菌Thermus thermophilus HB 8。噬菌体φ OH 3属于丝核病毒科，由长830 nm、宽8 nm的柔性丝状颗粒组成。φ OH 3在70 ~ 90°C和6 ~ 9的pH范围内稳定。噬菌体的一步生长曲线显示，感染后立即开始60分钟的潜伏期，随后在随后的40分钟内产生细胞内病毒颗粒。释放的φ OH 3病毒粒子数为109。在潜伏期期间，单链DNA（ssDNA）和噬菌体DNA的复制形式（RF）从40分钟开始倍增。在释放期间，ssDNA和RF DNA的拷贝数急剧增加。φ OH 3基因组全长5688 bp，有8个开放阅读框（ORF）。这些ORF在RF DNA的正链上编码，并且与任何已知的噬菌体基因没有显示出显著的同源性，除了ORF 5，其与栖热菌属丝状噬菌体PH 75的基因VIII产物显示出60%的同一性。所有ORF在氨基酸序列水平上与水生栖热菌Y51 MC 23和弱温菌DSM 17022基因组中注释的预测基因相似。这是首次报道一株丝状T.嗜热噬菌体在其宿主细胞内。
A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host. Phage φOH3, which was classified into the Inoviridae family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90°C and at pHs ranging from 6 to 9. A one-step growth curve of the phage showed a 60-min latent period beginning immediately postinfection, followed by intracellular virus particle production during the subsequent 40 min. The released virion number of φOH3 was 109. During the latent period, both single stranded DNA (ssDNA) and the replicative form (RF) of phage DNA were multiplied from min 40 onward. During the release period, the copy numbers of both ssDNA and RF DNA increased sharply. The size of the φOH3 genome is 5688 bp, and eight putative open reading frames (ORFs) were annotated. These ORFs were encoded on the plus strand of RF DNA and showed no significant homology with any known phage genes, except ORF 5, which showed 60% identity with the gene VIII product of the Thermus filamentous phage PH75. All the ORFs were similar to predicted genes annotated in the Thermus aquaticus Y51MC23 and Meiothermus timidus DSM 17022 genomes at the amino acid sequence level. This is the first report of the whole genome structure and DNA multiplication of a filamentous T. thermophilus phage within its host cell.