Sequence-Controlled Secondary Structures and Stimuli Responsiveness of Bioinspired Polyampholytes.
Sequence-Controlled Secondary Structures and Stimuli Responsiveness of Bioinspired Polyampholytes.
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仿生聚两性电解质的序列控制二级结构和刺激响应性。
DOI：
10.1021/acs.biomac.2c00666
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发表时间：
2022
影响因子：
6.2
通讯作者：
Tirrell,MatthewV
中科院分区：
文献类型：
--
作者：
Dinic,Jelena;Schnorenberg,MathewR;Tirrell,MatthewV
一个全面的研究，专注于退火的聚两性电解质的二级结构的偏好和它们对外界刺激的响应的序列电荷模式的影响。设计了两个序列，完全由可电离氨基酸（电荷分数，f= 1）和相同数量的正电荷和负电荷（f+=f-= 0.5）组成，具有由赖氨酸和谷氨酸单体组成的不同电荷模式。该研究表明，序列电荷模式有显着的影响，在生理pH值的聚两性电解质的二级结构偏好。此外，它表明，外部刺激，如pH值，离子强度，和溶剂介电常数可以用来调节二级结构的两个研究序列。所观察到的二级结构转换的两个序列也基本上不同于那些确定为均匀带电的同源多肽在匹配条件下。
A comprehensive study focusing on the influence of the sequence charge pattern on the secondary structure preferences of annealed polyampholytes and their responsiveness to external stimuli is presented. Two sequences are designed composed entirely of ionizable amino acids (charge fraction,f= 1) and an equal number of positive and negative charges (f+=f–= 0.5) with distinct charge patterns consisting of lysine and glutamic acid monomers. The study reveals that the sequence charge pattern has a significant influence on the secondary structure preferences of polyampholytes at physiological pH. Furthermore, it shows that external stimuli such as pH, ionic strength, and solvent dielectric constant can be used to modulate the secondary structure of the two studied sequences. The observed secondary structure transformations for the two sequences are also substantially different from those determined for uniformly charged homo-polypeptides under matching conditions.