Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice.
Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice.
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野生型和phyB突变体中microRNA及其靶标的全基因组鉴定提供了microRNA与水稻中phyB介导的光信号通路之间的关键联系
DOI：
10.3389/fpls.2015.00372
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发表时间：
2015
影响因子：
5.6
通讯作者：
Xie X
中科院分区：
文献类型：
--
作者：
Sun W;Xu XH;Wu X;Wang Y;Lu X;Sun H;Xie X
光敏色素B（phyB）是水稻光敏色素家族的一员，在水稻的一系列发育过程和逆境反应中起着重要的调控作用。然而，很少有信息参与的phyB介导的光信号通路的机制已在水稻中报道。microRNAs（miRNAs）在植物发育和逆境应答中也发挥着重要作用。因此，研究miRNAs在水稻phyB介导的光信号转导途径中的作用具有重要意义。在这项研究中，比较高通量测序和degradome分析被用来确定候选miRNAs和他们的目标，参与phyB介导的光信号通路。从野生型（WT）和phyB突变体水稻五叶期第四叶中共鉴定出720个已知miRNAs、704个新miRNAs和1957个靶基因。其中，135个miRNAs表达差异，提示这些miRNAs的表达直接或间接受phyB的调控。此外，在135个差异表达的miRNAs中，有32个被发现切割了水稻基因组中的70个基因。对这些靶基因的分析表明，各种转录因子家族的成员所占比例最大，表明miRNAs可能主要通过调控转录因子的表达参与phyB介导的光信号通路。这些结果为研究phyB介导的光信号通路中miRNAs的功能提供了新的线索，有助于全面揭示光敏色素介导的植物光形态建成和胁迫响应的分子机制。
Phytochrome B (phyB), a member of the phytochrome family in rice, plays important roles in regulating a range of developmental processes and stress responses. However, little information about the mechanisms involved in the phyB-mediated light signaling pathway has been reported in rice. MicroRNAs (miRNAs) also perform important roles in plant development and stress responses. Thus, it is intriguing to explore the role of miRNAs in the phyB-mediated light signaling pathway in rice. In this study, comparative high-throughput sequencing and degradome analysis were used to identify candidate miRNAs and their targets that participate in the phyB-mediated light signaling pathway. A total of 720 known miRNAs, 704 novel miRNAs and 1957 target genes were identified from the fourth leaves of wild-type (WT) and phyB mutant rice at the five-leaf stage. Among them, 135 miRNAs showed differential expression, suggesting that the expression of these miRNAs is directly or indirectly under the control of phyB. In addition, 32 out of the 135 differentially expressed miRNAs were found to slice 70 genes in the rice genome. Analysis of these target genes showed that members of various transcription factor families constituted the largest proportion, indicating miRNAs are probably involved in the phyB-mediated light signaling pathway mainly by regulating the expression of transcription factors. Our results provide new clues for functional characterization of miRNAs in the phyB-mediated light signaling pathway, which should be helpful in comprehensively uncovering the molecular mechanisms of phytochrome-mediated photomorphogenesis and stress responses in plants.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
6.4
作者：
Hao, Da-Cheng;Yang, Ling;Liu, Ming
通讯作者：
Liu, Ming
DOI：
10.1073/pnas.93.8.3530
发表时间：
1996-04-16
影响因子：
11.1
作者：
Carabelli, M;Morelli, G;Ruberti, I
通讯作者：
Ruberti, I
影响因子：
4.9
作者：
Correll, MJ;Kiss, JZ
通讯作者：
Kiss, JZ
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.