Internalization and degradation of heparin binding growth factor-I by endothelial cells.
Internalization and degradation of heparin binding growth factor-I by endothelial cells.
复制标题
内皮细胞对肝素结合生长因子-I 的内化和降解。
DOI：
10.1016/s0006-291x(88)80459-5
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Maciag,T
中科院分区：
文献类型：
--
作者：
Friesel,R;Maciag,T
用小鼠肺毛细血管内皮细胞（LEII）研究了~（125）I标记的肝素结合生长因子Ⅰ（~（125）I-HBGF-Ⅰ）与其细胞表面受体结合后的命运。在4°C下125 I-HBGF-I与其受体的结合显示pH依赖性，最佳结合在pH 6.5-7.5。在4°C下结合到细胞上的大部分（约80%）125 I-HBGF-I可以通过用低pH培养基洗涤而去除，但是当将细胞转移到37°C时，125 I-HBGF-I的50%在20分钟后变得耐酸。内化125 I-HBGF-I的电泳分析表明，内化后约2小时开始降解，出现Mr 15，000和Mr 10，000的两个主要标记片段。降解内化的125 I-HBGF-I被溶酶体促进剂氯喹抑制。这些数据表明，细胞相关的125 I-HBGF-I被迅速内化，并被引导到溶酶体细胞区室，在那里它被缓慢降解。
The fate of125I-labeled heparin binding growth factor I (125I-HBGF-I) after binding to its cell surface receptor has been studied using murine lung capillary endothelial cells (LEII). Binding of125I-HBGF-I to its receptor at 4°C shows pH dependence with optimal binding at pH 6.5–7.5. The majority (∼80%) of125I-HBGF-I bound to cells at 4°C can be removed by washing with low pH medium, but rapidly becomes acid resistant upon shifting cells to 37°C, with 50% of the125I-HBGF-I becoming acid resistant after 20 minutes. Electrophoretic analysis of internalized125I-HBGF-I shows that degradation begins approximately 2 hours after internalization with the appearance of two major labeled fragments of Mr15,000 and Mr10,000. Degradation of internalized125I-HBGF-I is inhibited by the lysosomotropic agent chloroquine. These data suggest that cell-associated125I-HBGF-I is rapidly internalized and directed to a lysosomal cellular compartment where it is slowly degraded.