Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats.
Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats.
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DOI：
10.1016/j.mce.2009.02.001
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发表时间：
2009-05-06
影响因子：
4.1
通讯作者：
Davies KP
中科院分区：
文献类型：
--
作者：
Chua RG;Calenda G;Zhang X;Siragusa J;Tong Y;Tar M;Aydin M;DiSanto ME;Melman A;Davies KP
Vcsa1在大鼠勃起生理中起重要作用。我们进行了实验，以确定勃起功能、睾酮水平和Vcsa1表达是否相关。去睾丸大鼠血中总睾酮从平均4 ng/ml降至0.04 ng/ml。勃起功能显著低于对照组，Vcsa1表达显著降低(~gt；6倍)。睾丸切除动物注射睾酮(每隔4天注入2毫克100毫升芝麻油，持续两周)，使睾酮平均水平恢复到2 ng/ml，增强勃起功能，并显著增加Vcsa1的表达。在分离的体细胞中，存在睾酮依赖的Vcsa1表达。然而，在切除的动物体内注射表达Vcsa1或其基因产物西乐洛芬(先前证明可以改善老年动物的勃起功能)的质粒并没有显著改善勃起功能。此外，与对照组相比，西拉洛芬降低从切除动物分离的体部肌条张力的能力受到损害。
Vcsa1 plays an important role in the erectile physiology of the rat. We conducted experiments to determine if erectile function, testosterone levels and Vcsa1 expression were correlated. In orchiectomized rats, total testosterone in blood fell from an average of 4ng/ml to <0.04ng/ml. Erectile function was significantly lower compared to controls and Vcsa1 expression was significantly (>6-fold) decreased. Injection of orchiectomized animals with testosterone (2mg in 100ml sesame oil every 4 days for two weeks) restored average levels of testosterone to 2ng/ml, increased erectile function and significantly increased Vcsa1 expression. In isolated corporal cells there was testosterone dependent Vcsa1 expression. However, intracorporal injection of orchiectomized animals with a plasmid expressing Vcsa1 or its gene product Sialorphin (previously demonstrated to improve erectile function in old animals) gave no significant improvement in erectile function. Also, the ability of Sialorphin to reduce tension in corporal smooth muscle strips isolated from orchiectomized animals was impaired compared to controls.
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DOI：
10.1152/ajpheart.1998.275.2.h600
发表时间：
1998-08-01
影响因子：
4.8
作者：
Christ, GJ;Rehman, J;Geliebter, J
通讯作者：
Geliebter, J
影响因子：
3.5
作者：
Han, Deok Hyun;Chae, Mee Ree;Lee, Sung Won
通讯作者：
Lee, Sung Won
影响因子：
4.8
作者：
Rosinski-Chupin, I;Huaulmé, JF;Rougeon, F
通讯作者：
Rougeon, F
影响因子：
5.5
作者：
Zabka, AG;Mitchell, GS;Behan, A
通讯作者：
Behan, A
DOI：
10.1016/s0960-0760(01)00107-8
发表时间：
2001-10-01
影响因子：
4.1
作者：
Banu, KS;Govindarajulu, P;Aruldhas, MM
通讯作者：
Aruldhas, MM