The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs
The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs
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5-羟色胺、5-羟色氨酸和利血平对麻醉豚鼠肠道蠕动的作用
DOI：
10.1113/jphysiol.1959.sp006176
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发表时间：
1959
期刊：
影响因子：
--
通讯作者：
A. Crema
中科院分区：
文献类型：
--
作者：
E. Bülbring;A. Crema
方法正常豚鼠于实验前1h静脉注射氯醛糖-氨基甲酸乙酯(0.6%氯醛糖加入10%氨基甲酸乙酯)1ml./100g。利血平治疗的豚鼠只耐受正常麻醉剂剂量的一半，没有呼吸停止，为了避免这种情况，他们被皮下注射。由于手术麻醉通常不能获得，所以用普鲁卡因渗透腹部皮肤。这还有一个额外的好处，可以防止心脏颤动，心脏颤动被发现是这些动物的死亡原因。利血平治疗的豚鼠在整个实验过程中都进行了人工呼吸。在活体内灌流一个肠环的实验安排已经在前面描述过了(Biillining&Crema，1959)。在本研究中，在许多实验中使用了两个肠环。这只动物被放在加热的桌子上，由红外线灯从上方加热，使温度保持在380摄氏度。腹部在中线切开，切下两段5-8厘米长的回肠，保留它们的肠系膜和血液供应。它们身上覆盖着薄纱，并通过不断滴下温暖的蒂罗德水来保持湿润。其中一块作为对照，在不同的实验中，它交替地比另一块更多地口服或流产。液体通过两个血管的管腔。它们连接到同一个储水池，但通过一对阀门相互隔离，因此两个环路的灌流压力相同，但一个环路的收缩不能改变另一个环路的压力。
METHODSNormal guinea-pigs were anaesthetized by intravenous injection of chloralose-urethane (0.6% chloralose in 10% urethane), 1 ml./100 g, one hour before the experiment. Reserpine-treated guinea-pigs tolerated only half of the normal anaesthetic dose without respiratory arrest, and in order to avoid this they were injected subcutaneously. As surgical anaesthesia was usually not obtained the skin of the abdomen was infiltrated with procaine. This had the additional advantage of preventing cardiac fibrillation which was found to be a cause of death in these animals. The reserpine-treated guinea-pigs were given artificial respiration throughout the experiment. The experimental arrangement for perfusing one loop of intestine in vivo has been described previously (Biilbring & Crema, 1959). In the present investigation two loops of intestine were used in many experiments. The animal was put on its back on a heated table and warmed from above by an infra-red lamp keeping the temperature constant at 380 C. The abdomen was opened in the mid line and two pieces of ileum of 5-8 cm length-were cut, leaving their mesentery and blood supply intact. They were covered with muslin and kept moist by a constant drip of warm Tyrode. One piece served as control, and in different experiments it was alternately more oral or aboral than the otherpiece. Fluid was passed through the lumen of both loops. They were connected to the same reservoir, but isolated from each other by a pair of valves so that the perfusion pressure for the two loops was the same, but the contractions of one loop could not change the pressure in the other.