Contributions of white and brown adipose tissues and skeletal muscles to acute cold-induced metabolic responses in healthy men
Contributions of white and brown adipose tissues and skeletal muscles to acute cold-induced metabolic responses in healthy men
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DOI：
10.1113/jphysiol.2014.283598
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发表时间：
2015-02-01
影响因子：
5.5
通讯作者：
Haman, Francois
中科院分区：
文献类型：
--
作者：
Blondin, Denis P.;Labbe, Sebastien M.;Haman, Francois
要点棕色脂肪组织（BAT）和骨骼肌激活都有助于健康男性急性寒冷暴露的代谢反应，即使在最轻微的颤抖下也是如此。健康男性急性冷暴露时脂肪组织细胞内脂解的激活与BAT代谢反应相关。虽然BAT葡萄糖摄取单位体积的组织是重要的，在冷暴露过程中的葡萄糖周转介导的骨骼肌代谢活化，即使颤抖是最小化。冷暴露刺激交感神经系统（SNS），触发激活冷防御反应和动员底物燃料产热过程。虽然这些过程已被独立研究，生理相互作用和协调的组织参与产生热量或动员底物的贡献从来没有在人类中进行过研究。使用[U-C-13]-棕榈酸盐和[3-H-3]-葡萄糖示踪剂方法，结合使用C-11-乙酸盐和F-18-氟脱氧葡萄糖的正电子发射断层扫描，我们检查了全身交感神经诱导的白色脂肪组织（WAT）脂解和棕色脂肪组织（BAT）代谢之间的关系，并绘制了轻度，急性寒冷暴露，旨在减少12名瘦健康男性的颤抖反应。冷诱导的全身耗氧量增加与BAT活动量、BAT氧化代谢或肌肉代谢或颤抖强度无关，但取决于这两种代谢组织的反应总和。冷诱导的非酯化脂肪酸（NEFA）出现率的增加与代谢活性BAT的体积（r=0.80，P=0.005）、总BAT氧化代谢（r=0.70，P=0.004）和BAT葡萄糖摄取（r=0.80，P=0.005）密切相关，但与肌肉葡萄糖代谢无关。在冷暴露期间，骨骼肌的总葡萄糖摄取量比BAT高出一个数量级以上（674 +/-124 vs. 12 ± 8molmin（-1），P
Key points Both brown adipose tissue (BAT) and skeletal muscle activation contribute to the metabolic response of acute cold exposure in healthy men even under minimal shivering. Activation of adipose tissue intracellular lipolysis is associated with BAT metabolic response upon acute cold exposure in healthy men. Although BAT glucose uptake per volume of tissue is important, the bulk of glucose turnover during cold exposure is mediated by skeletal muscle metabolic activation even when shivering is minimized.Cold exposure stimulates the sympathetic nervous system (SNS), triggering the activation of cold-defence responses and mobilizing substrates to fuel the thermogenic processes. Although these processes have been investigated independently, the physiological interaction and coordinated contribution of the tissues involved in producing heat or mobilizing substrates has never been investigated in humans. Using [U-C-13]-palmitate and [3-H-3]-glucose tracer methodologies coupled with positron emission tomography using C-11-acetate and F-18-fluorodeoxyglucose, we examined the relationship between whole body sympathetically induced white adipose tissue (WAT) lipolysis and brown adipose tissue (BAT) metabolism and mapped the skeletal muscle shivering and metabolic activation pattern during a mild, acute cold exposure designed to minimize shivering response in 12 lean healthy men. Cold-induced increase in whole-body oxygen consumption was not independently associated with BAT volume of activity, BAT oxidative metabolism, or muscle metabolism or shivering intensity, but depended on the sum of responses of these two metabolic tissues. Cold-induced increase in non-esterified fatty acid (NEFA) appearance rate was strongly associated with the volume of metabolically active BAT (r=0.80, P=0.005), total BAT oxidative metabolism (r=0.70, P=0.004) and BAT glucose uptake (r=0.80, P=0.005), but not muscle glucose metabolism. The total glucose uptake was more than one order of magnitude greater in skeletal muscles compared to BAT during cold exposure (674 +/- 124vs. 12 +/- 8molmin(-1), respectively, P