Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
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DOI：
10.7554/elife.17879
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发表时间：
2016-09-22
期刊：
影响因子：
7.7
通讯作者：
Garrity, Paul A.
中科院分区：
文献类型：
--
作者：
Knecht, Zachary A.;Silbering, Ana F.;Garrity, Paul A.
离子型受体（IR）是存在于原口动物中的变体离子型谷氨酸受体的一个大的亚家族。虽然这些受体在化学感觉检测中的作用得到了最广泛的研究，但最近的工作涉及两个家族成员，IR 21 a和IR 25 a，在果蝇的温度感觉中。在这里，我们描述了进化上最高度保守的受体之一IR 93 a的特征，并表明它与IR 21 a和IR 25 a共表达并发挥作用，介导对低温的生理和行为反应。IR93 a还与IR25 a和一种独特的受体IR40 a共表达于球囊（saculus）中的感觉神经元的离散群体中，球囊是天线内的多室口袋。我们证明，这种受体的组合是必要的神经元对干燥空气和湿度差异的行为歧视的反应。我们的研究结果确定IR93a作为一个共同的组成部分，分子和细胞不同的IR途径重要的温度和湿度的昆虫。
lonotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these receptors are most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, in thermosensation in Drosophila. Here we characterize one of the most evolutionarily deeply conserved receptors, IR93a, and show that it is co-expressed and functions with IR21a and IR25a to mediate physiological and behavioral responses to cool temperatures. IR93a is also co-expressed with IR25a and a distinct receptor, IR40a, in a discrete population of sensory neurons in the sacculus, a multi-chambered pocket within the antenna. We demonstrate that this combination of receptors is required for neuronal responses to dry air and behavioral discrimination of humidity differences. Our results identify IR93a as a common component of molecularly and cellularly distinct IR pathways important for thermosensation and hygrosensation in insects.