BIODEGRADATION OF POLYCYCLIC AROMATIC-HYDROCARBONS BY PHANEROCHAETE-CHRYSOSPORIUM
BIODEGRADATION OF POLYCYCLIC AROMATIC-HYDROCARBONS BY PHANEROCHAETE-CHRYSOSPORIUM
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DOI：
10.1128/aem.55.1.154-158.1989
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发表时间：
1989-01-01
影响因子：
4.4
通讯作者：
BUMPUS, JA
中科院分区：
文献类型：
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作者：
BUMPUS, JA
证明了白腐真菌 Phanerochaete chrysosporium 能够降解蒽油（从煤焦油中获得的蒸馏产品）中存在的多环芳烃 (PAH)。毛细管气相色谱和高效液相色谱分析表明，在该真菌的营养氮限制培养物培养 27 天期间，至少 22 种多环芳烃（包括蒽油中存在的所有最丰富的多环芳烃成分）消失了 70% 至 100%。由于菲是蒽油中含量最丰富的多环芳烃，因此选择该多环芳烃进行进一步研究。在将[ 14 C]菲与含有蒽油的黄孢青霉培养物一起培养27天的实验中，结果表明，最初存在于[ 14 C]菲中的回收的放射性标记碳的7.7％被代谢为14CO 2 ，并且从水性部分中回收了25.2％，而从二氯甲烷和二氯甲烷中回收了56.1％和11.0％。 分别为颗粒部分。对二氯甲烷馏分中存在的 14C 标记材料进行高效液相色谱分析表明，该材料的大部分 (91.9%) 由 [14C] 菲的极性代谢物组成。这些结果表明，这种微生物可能有助于净化环境中受多环芳烃污染的场所。
The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons (PAHs) that are present in anthracene oil (a distillation product obtained from coal tar) was demonstrated. Analysis by capillary gas chromatography and high-performance liquid chromatography showed that at least 22 PAHs, including all of the most abundant PAH components present in anthracene oil, underwent 70 to 100% disappearance during 27 days of incubation with nutrient nitrogen-limited cultures of this fungus. Because phenanthrene is the most abundant PAH present in anthracene oil, this PAH was selected for further study. In experiments in which [14C]phenanthrene was incubated with cultures of P. chrysosporium containing anthracene oil for 27 days, it was shown that 7.7% of the recovered radiolabeled carbon originally present in [14C]phenanthrene was metabolized to 14CO2 and 25.2% was recovered from the aqueous fraction, while 56.1 and 11.0% were recovered from the methylene chloride and particulate fractions, respectively. High-performance liquid chromatography of the 14C-labeled material present in the methylene chloride fraction revealed that most (91.9%) of this material was composed of polar metabolities of [14C]phenanthrene. These results suggest that this microorganism may be useful for the decontamination of sites in the environment contaminated with PAHs.