Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
Detection of progesterone in aqueous samples by molecularly imprinted photonic polymers
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DOI：
10.1007/s00604-022-05290-w
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发表时间：
2022-05-01
影响因子：
5.7
通讯作者：
Fidalgo, Maria
中科院分区：
文献类型：
--
作者：
Qasim, Sally;Hsu, Shu-Yu;Fidalgo, Maria
通过在胶体晶体的空隙空间内聚合，使其具有光子特性，制备了一种用于检测水溶液中孕酮的无标记分子印迹聚合物（MIP）传感器。以丙烯酸为功能单体，乙二醇为交联剂，乙醇为溶剂，黄体酮为印迹模板，制备了预聚合物。聚合后，通过酸蚀刻除去胶体晶体，用溶剂洗脱靶。材料表征包括如下：衰减全反射傅里叶变换红外光谱，动态光散射，溶胀实验，和环境扫描电子显微镜。通过平衡结合、动力学实验和紫外-可见光谱研究了MIPs，以研究在去离子水和150 mM NaCl溶液中不同孕酮浓度下发生的再结合的布拉格衍射峰位移。研究了孕酮浓度在1-100 μ g L-1范围内的MIP响应，LOD为0.5 μ g L-1，达到了天然沃茨中激素的检测范围。此外，水凝胶MIP膜在各种真实的水基质中成功地进行了测试，结果令人满意。此外，MIP膜表现出良好的选择性对孕激素证明了一个更大的响应比当暴露于结构相似的分子。计算研究表明，尺寸沿着与表面电位影响类似化合物的结合。由于其易于使用和低成本，传感器是有前途的筛选工具，在水溶液样品中的孕酮的存在。
A label-free molecular imprinted polymer (MIP) sensor was fabricated for the detection of progesterone in aqueous solutions, by polymerization inside the void spaces of colloidal crystals, which gave them photonic properties. The prepolymerization mixture was prepared from acrylic acid as the functional monomer, ethylene glycol as the cross-linker agent, ethanol as solvent, and progesterone as the imprinted template. After polymerization, the colloidal crystal was removed by acid etching and the target eluted with a solvent. Material characterization included as follows: attenuated total reflectance-Fourier-transform infrared spectroscopy, dynamic light scattering, swelling experiments, and environmental scanning electron microscopy. MIPs were investigated by equilibrium binding, kinetics experiments, and UV-visible spectra to investigate Bragg diffraction peak shift that occurs with the rebinding at different progesterone concentrations in deionized water and 150-mM NaCl solutions. The MIP response was investigated with progesterone concentration in the 1-100 mu g L-1 range, with LOD of 0.5 mu g L-1, reaching the detected range of hormone in natural waters. Furthermore, hydrogel MIP films were successfully tested in various real water matrices with satisfactory results. Moreover, the MIP film exhibited good selectivity toward the progesterone hormone evidenced by a larger response than when exposed to structurally similar molecules. Computational studies suggested that size along with surface potential influenced the binding of analog compounds. Due to their ease of use and low cost, the sensors are promising as screening tools for the presence of progesterone in aqueous samples.