IL-17A plays an important role in protection induced by vaccination with fibronectin-binding domain of fibronectin-binding protein A against Staphylococcus aureus infection
IL-17A plays an important role in protection induced by vaccination with fibronectin-binding domain of fibronectin-binding protein A against Staphylococcus aureus infection
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DOI：
10.1007/s00430-017-0499-9
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发表时间：
2017-06-01
影响因子：
5.4
通讯作者：
Nakane, Akio
中科院分区：
文献类型：
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作者：
Narita, Kouji;Asano, Krisana;Nakane, Akio
金黄色葡萄球菌纤连蛋白结合蛋白A（Fibronectin-binding protein A，FnBPA）是金黄色葡萄球菌的重要毒力因子之一。金黄色葡萄球菌感染。因此，它被认为是潜在的候选疫苗。先前的研究已经报道，接种FnBPA可以保护动物免受S。金黄色葡萄球菌感染在这项研究中，我们证明了用FnBPA的纤连蛋白结合结构域（FnBPA（541-870））接种疫苗可以保护野生型小鼠而不是白细胞介素-17A（IL-17 A）缺陷型小鼠对抗S。金黄色葡萄球菌感染。在接种野生型和IL-17 A缺陷型小鼠的血清中产生中等水平的抗原特异性免疫球蛋白。免疫小鼠的脾细胞通过抗原刺激产生IL-17 A，并且在感染后免疫小鼠的脾和肝中IL-17 A mRNA表达增加。感染后，免疫小鼠脾脏中CXCL 1和CXCL 2 mRNA表达增加，脾脏和肝脏中髓过氧化物酶（MPO）活性增加。这些结果表明，用FnBPA（541-870）接种诱导产生IL-17 A的细胞，并且IL-17 A介导的细胞免疫参与对S.金黄色葡萄球菌感染。
Fibronectin-binding protein A (FnBPA) of Staphylococcus aureus is a microbial surface component recognizing adhesive matrix molecules and has been known as one of the most important virulence factors involved in the initiation step of S. aureus infection. Therefore, it has been considered as a potential vaccine candidate. Previous studies have reported that vaccination with FnBPA protects animals against S. aureus infection. In this study, we demonstrated that vaccination with fibronectin-binding domain of FnBPA (FnBPA(541-870)) protects wild-type mice but not interleukin-17A (IL-17A)-deficient mice against S. aureus infection. Moderate levels of antigen-specific immunoglobulins were produced in the sera of vaccinated wild-type and IL-17A-deficient mice. The spleen cells of vaccinated mice produced IL-17A by stimulation with the antigen, and IL-17A mRNA expression was increased in the spleens and livers of vaccinated mice after infection. CXCL1 and CXCL2 mRNA expression was increased in the spleens, and myeloperoxidase (MPO) activity in the spleens and livers was increased in the vaccinated mice after infection. These results suggest that vaccination with FnBPA(541-870) induces the IL-17A-producing cells and that IL-17A-mediated cellular immunity is involved in the protective effect on S. aureus infection.