Thermal self-regulatory smart biosensor based on horseradish peroxidase-immobilized phase-change microcapsules for enhancing detection of hazardous substances
Thermal self-regulatory smart biosensor based on horseradish peroxidase-immobilized phase-change microcapsules for enhancing detection of hazardous substances
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基于辣根过氧化物酶固定相变微胶囊的热自调节智能生物传感器增强有害物质检测
DOI：
10.1016/j.cej.2021.132982
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发表时间：
2021-10
影响因子：
15.1
通讯作者：
Wang Xiaodong
中科院分区：
文献类型：
--
作者：
Xiong Jian;Sun Zhao;Yu Jinghua;Liu Huan;Wang Xiaodong
由于生物催化活性对温度有很强的依赖性，传统的基于酶的生物传感器对操作温度高度敏感。为了提高高温环境下有害物质的生物传感检测能力，我们将相变材料(PCM)与生物电催化材料相结合，设计并构建了一种热自适应智能生物传感器。首先将正二十烷作为相变微囊的核心微胶囊包裹在二氧化钛壳层中，然后在二氧化钛壳层表面沉积聚吡咯(PPy)作为电活性涂层，然后通过物理吸附将辣根过氧化物酶固定在聚吡咯涂层表面，制备了生物电催化相变微胶囊体系。所得到的微胶囊具有规则的球形和层层核壳结构，具有所需的化学成分。该微胶囊不仅表现出良好的热管理能力，在潜热容量约为115×J/g的情况下进行有效的温度调节，而且具有较高的抗热冲击能力和良好的热循环稳定性，适用于生物传感器的长期热管理应用。用上述微胶囊修饰工作电极，构建了具有热自调节能力的电化学生物传感系统。在55℃下，该智能生物传感器的灵敏度为5.571µA·L·−1·cm−2，检测下限为5.384µm ol/L，由于其正二十烷核心的就地热管理，在高温下比传统生物传感器具有更好的检测邻苯二酚的能力。这项研究为开发具有增强识别能力的热自调节智能生物传感器提供了一种新的方法，能够在广泛的温度范围内检测危险物质。
Conventional enzyme-based biosensors are highly sensitive to operation temperature due to a strong temperature dependency of biocatalytic activity. Aiming to enhance the biosensing detection of hazardous substances at high ambient temperatures, we focused on the design and construction of a thermal self-regulatory smart biosensor through an innovative combination of phase change material (PCM) and bioelectrocatalytic material. A bioelectrocatalytic phase-change microcapsule system was first fabricated by microencapsulatingn-eicosane as a PCM core in the TiO2shell and then depositing polypyrrole (PPy) as an electroactive coating layer on the surface of TiO2shell, followed by immobilizing horseradish peroxidase on the surface of PPy coating layer through physical adsorption. The resultant microcapsules exhibit a regular spherical morphology and layer-by-layer core–shell microstructure with the desired chemical compositions. The microcapsules not only exhibit a good thermal management ability to perform effective temperature regulation under a latent-heat capacity of approximately 115 J/g, but also reveal high thermal impact resistance and good thermal cycle stability for the long-term thermal management application in biosensors. A working electrode was modified with the microcapsules obtained above and then used to construct an electrochemical biosensing system imparted with a thermal self-regulation capability. With a high sensitivity of 5.571 µA·L·µmol−1·cm−2and a low detection limit of 5.384 µmol/L at 55 °C, the resultant smart biosensor exhibits a better determination ability to detect catechol as a model hazardous substance at high operation temperatures than conventional biosensors thanks to thein-situthermal management derived from itsn-eicosane core. This study provides a new approach for development of thermal self-regulatory smart biosensors with an enhanced identification capability to detect hazardous substances over a wide range of temperatures.
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影响因子：
9.5
作者：
Chan DH;Millet A;Fisher CR;Price MC;Burchell MJ;Armes SP
通讯作者：
Armes SP
影响因子：
6.6
作者：
Tong Wu;Guiru Sun;W. Lu;Liping Zhao;A. Mauger;C. Julien;Liqun Sun;Haiming Xie;Jinghai Liu
通讯作者：
Tong Wu;Guiru Sun;W. Lu;Liping Zhao;A. Mauger;C. Julien;Liqun Sun;Haiming Xie;Jinghai Liu
影响因子：
6.2
作者：
Hernández-Cancel G;Suazo-Dávila D;Medina-Guzmán J;Rosado-González M;Díaz-Vázquez LM;Griebenow K
通讯作者：
Griebenow K
微胶囊相变材料智能电极设计对柔性超级电容器的原位温度调控
影响因子：
6.6
作者：
Xiao-ying Xu;Jia-hua Liu;Xing Ouyang;Lifeng Cui;Jiao-ling Hong;Xiao Meng;Siyin Qin;Chen Liu;Jiaoning Tang;Dazhu Chen
通讯作者：
Dazhu Chen
具有自组织表面结构的多层聚吡咯纳米片可实现灵活高效的太阳能-热能转换
影响因子：
29.4
作者：
Wang, Xu;Liu, Qingchang;Xu, Hangxun
通讯作者：
Xu, Hangxun