THE ERYTHROID-SPECIFIC TRANSCRIPTION FACTOR ERYF1 - A NEW FINGER PROTEIN
THE ERYTHROID-SPECIFIC TRANSCRIPTION FACTOR ERYF1 - A NEW FINGER PROTEIN
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DOI：
10.1016/0092-8674(89)90940-9
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发表时间：
1989-09-08
期刊：
影响因子：
64.5
通讯作者：
FELSENFELD, G
中科院分区：
文献类型：
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作者：
EVANS, T;FELSENFELD, G
红系特异性转录因子Eryf1与鸡的α-和β-珠蛋白家族每个成员的调控区域内的DNA位点结合。这些位点的分布表明，Eryf1可能作为红系发育的一种通用“开关”因子。我们已经克隆了Eryf1的cDNA，并表明相应的mRNA存在于所有红系谱系中，但在非红系细胞中不存在。我们证明该cDNA编码在红细胞中发现的特异性Eryf1结合活性。Eryf1是一种分子量为38kD的碱性蛋白，包含一对高度相似的“指状结构”，其基序为Cys - x - x - Cys - x17 - Cys - x - x - Cys。这些区域的氨基酸序列与其他具有类似半胱氨酸残基排列的调控蛋白中的序列没有相似之处。此外，我们的证据表明，过渡金属离子异常紧密地结合，或者对于Eryf1的序列特异性DNA结合可能不是必需的。
The erythroid-specific transcription factor Eryf1 binds to DNA sites within regulatory regions of every member of both the .alpha.- and .beta.-globin families in chicken. The distribution of these sites suggests that Eryf1 may serve as a general "switch" factor for erythroid development. We have cloned the cDNA for Eryf1 and show that the corresponding mRNA is present in all erythroid lineages, but is absent from non-erythroid cells. We demonstrate that the cDNA encodes the specific Eryf1 binding activity found in erythrocytes. Eryf1 is a basic 38 kd protein containing a pair of highly similar "fingers" with the motif Cys-x-x-Cys-x17-Cys-x-x-Cys. The amino acid sequences of these regions bear no resemblance to those found in other regulatory proteins with a similar arrangement of cysteine residues. Our evidence suggests, furthermore, that transition metal ions are unusually tightly bound, or may not be necessary for the sequence-specific DNA binding of Eryf1.