Mini-array of multiple tumor-associated antigens (TAAs) in the immunodiagnosis of esophageal cancer.
Mini-array of multiple tumor-associated antigens (TAAs) in the immunodiagnosis of esophageal cancer.
复制标题
DOI：
10.7314/apjcp.2014.15.6.2635
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Jiejie Qin;Xiao-Rui Wang;Peng Wang;P. Ren;Jianxiang Shi;Hong-fei Zhang;J. Xia;Kaijuan Wang;
中科院分区：
文献类型：
--
作者：
Jiejie Qin;Xiao-Rui Wang;Peng Wang;P. Ren;Jianxiang Shi;Hong-fei Zhang;J. Xia;Kaijuan Wang;
癌症患者的血清可能含有与称为肿瘤相关抗原（TAA）的独特组的自体细胞抗原反应的抗体。本研究旨在确定多个TAAs的微型阵列是否会增强抗体检测，并成为食管癌检测和诊断的有用方法。我们的多TAA微阵列由11种抗原组成，p53，p16，Impl，CyclinB 1，C-myc，RalA，p62，Survivin，Koc，CyclinD 1和CyclinE全长重组蛋白。应用酶联免疫吸附试验（ELISA）检测了174例食管癌患者和242例正常人血清中11种TAAs的自身抗体。Western blotting证实ELISA检测结果为阳性。在平行筛选试验中，随着抗原的连续添加，最终总共11个TAA，阳性抗体反应逐步增加。11种TAA为最佳平行组合，诊断食管癌的敏感性和特异性分别为75.3%和81.0%。阳性预测值为74.0%，阴性预测值为82.0%，表明11种TAAs的平行检测显著提高了诊断的准确性。另外，食管癌患者血清中抗p53、Impl、C-myc、RalA、p62、Survivin和CyclinD 1 7种抗原的抗体水平在不同分期间差异显著，提示自身抗体可能参与了食管癌的发生和发展。总而言之，这项研究进一步支持了我们之前的假设，即抗体的组合可能对某些类型的癌症的诊断具有更高的灵敏度。一个定制的多个精心选择的TAAs的微型阵列能够提高自身抗体检测在食管癌的免疫诊断和TAAs的自身抗体可能是临床分期的参考指标。
Sera of cancer patients may contain antibodies that react with a unique group of autologous cellular antigens called tumor-associated antigens (TAAs). The present study aimed to determine whether a mini-array of multiple TAAs would enhance antibody detection and be a useful approach in esophageal cancer detection and diagnosis. Our mini-array of multiple TAAs consisted of eleven antigens, p53, pl6, Impl, CyclinB1, C-myc, RalA, p62, Survivin, Koc, CyclinD1 and CyclinE full-length recombinant proteins. Enzyme-linked immunosorbent assays (ELISA) were used to detect autoantibodies against eleven selected TAAs in 174 sera from patients with esophageal cancer, as well as 242 sera from normal individuals. In addition, positive results of ELISA were confirmed by Western blotting. In a parallel screening trial, with the successive addition of antigen to a final total of eleven TAAs, there was a stepwise increase in positive antibody reactions. The eleven TAAs were the best parallel combination, and the sensitivity and specificity in diagnosing esophageal cancer was 75.3% and 81.0%, respectively. The positive and negative predictive values were 74.0% and 82.0%, respectively, indicating that the parallel assay of eleven TAAs raised the diagnostic precision significantly. In addition, the levels of antibodies to seven antigens, comprising p53, Impl, C-myc, RalA, p62, Survivin, and CyclinD1, were significantly different in various stages of esophageal cancer, which showed that autoantibodies may be involved in the pathogenesis and progression of esophageal cancer. All in all, this study further supports our previous hypothesis that a combination of antibodies might acquire higher sensitivity for the diagnosis of certain types of cancer. A customized mini-array of multiple carefully-selected TAAs is able to enhance autoantibody detection in the immunodiagnosis of esophageal cancer and autoantibodies to TAAs might be reference indicators of clinical stage.