Role of NF-Y in in vivo regulation of the γ-globin gene
Role of NF-Y in in vivo regulation of the γ-globin gene
复制标题
DOI：
10.1128/mcb.21.9.3083-3095.2001
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发表时间：
2001-05-01
影响因子：
5.3
通讯作者：
Li, QL
中科院分区：
文献类型：
--
作者：
Duan, ZJ;Stamatoyannopoulos, G;Li, QL
复制的CCAAT盒是γ基因表达所必需的。我们在这里报道，转录因子NF-Y被招募到体内复制的CCAAT盒子中。严重破坏NF-Y结合的重复CCAAT盒突变也降低了γ基因启动子的可及性水平，影响了基础转录机制的组装，增加了GATA-1向基因座中心区域（LCR）和近端启动子的募集，以及转录辅助因子CBP/p300向LCR的募集。这些发现表明，NF-Y被募集到复制的CCAAT盒子中，在γ基因启动子的染色质打开以及γ基因启动子与LCR之间的通信中发挥了作用。
The duplicated CCAAT box is required for gamma gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus central region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR.