Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil
Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil
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DOI：
10.1007/s00122-012-1849-z
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发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
Bilyeu, Kristin D.
中科院分区：
文献类型：
--
作者：
Anh-Tung Pham;Shannon, J. Grover;Bilyeu, Kristin D.
利用突变的FAD2-1A和FAD2-1B基因组合，获得了高油酸大豆。尽管这些大豆的油酸含量很高，但亚麻酸的含量在4-6%的范围内，这可能足以导致油脂的氧化不稳定。因此，本研究将一个或两个突变的FAD3基因整合到高油酸背景中，以进一步降低亚麻酸的含量。因此，利用不同来源的突变FAD2-1A基因，获得了高油酸、低亚麻酸含量的大豆品系。虽然油酸含量在两个测试环境中都是稳定的，但亚麻酸含量的降低取决于突变的FAD3基因和突变的FAD2-1基因的数量、FAD2-1A基因突变的严重程度以及测试环境。两个突变的FAD2-1基因和一个突变的FAD3基因的组合导致密苏里州波塔格维尔的亚麻酸含量低于2%，而密苏里州哥伦比亚市需要四个突变基因才能获得相同的亚麻酸含量。这项研究培育出了迄今为止报道的油酸含量最高、亚麻酸含量最低的非转基因大豆，为生产类似橄榄油的脂肪酸提供了一种独特的替代方案。
High oleic acid soybeans were produced by combining mutant FAD2-1A and FAD2-1B genes. Despite having a high oleic acid content, the linolenic acid content of these soybeans was in the range of 4-6 %, which may be high enough to cause oxidative instability of the oil. Therefore, a study was conducted to incorporate one or two mutant FAD3 genes into the high oleic acid background to further reduce the linolenic acid content. As a result, soybean lines with high oleic acid and low linolenic acid (HOLL) content were produced using different sources of mutant FAD2-1A genes. While oleic acid content of these HOLL lines was stable across two testing environments, the reduction of linolenic acid content varied depending on the number of mutant FAD3 genes combined with mutant FAD2-1 genes, on the severity of mutation in the FAD2-1A gene, and on the testing environment. Combination of two mutant FAD2-1 genes and one mutant FAD3 gene resulted in less than 2 % linolenic acid content in Portageville, Missouri (MO) while four mutant genes were needed to achieve the same linolenic acid in Columbia, MO. This study generated non-transgenic soybeans with the highest oleic acid content and lowest linolenic acid content reported to date, offering a unique alternative to produce a fatty acid profile similar to olive oil.