EXPRESSION CLONING OF SIAMOIS, A XENOPUS HOMEOBOX GENE EXPRESSED IN DORSAL VEGETAL CELLS OF BLASTULAS AND ABLE TO INDUCE A COMPLETE SECONDARY AXIS
EXPRESSION CLONING OF SIAMOIS, A XENOPUS HOMEOBOX GENE EXPRESSED IN DORSAL VEGETAL CELLS OF BLASTULAS AND ABLE TO INDUCE A COMPLETE SECONDARY AXIS
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DOI：
10.1016/0092-8674(95)90373-9
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发表时间：
1995-04-07
期刊：
影响因子：
64.5
通讯作者：
GURDON, JB
中科院分区：
文献类型：
--
作者：
LEMAIRE, P;GARRETT, N;GURDON, JB
使用的表达克隆策略，依赖于功能测定，我们已经克隆了一种新的非洲爪蟾含同源框基因，Siamois。胚胎注入腹植物卵裂球与少至5 pg的Siamois mRNA开发一个完整的第二轴，但注入细胞的后代不参与第二轴的形成。在正常发育中，Siamois mRNA首先在中胚转变后不久检测到，这比goosecoid或Xbrachyury的mRNA更早，并且在早期原肠胚的背内胚层中存在最丰富。该基因的激活可以在分散的胚胎细胞中自主获得。这些结果表明Siamois可能在Nieuwkoop中心的形成中起重要作用。
Using an expression cloning strategy that relies on a functional assay, we have cloned a novel Xenopus homeobox-containing gene, Siamois. Embryos injected in a ventral-vegetal blastomere with as little as 5 pg of Siamois mRNA develop a complete secondary axis, but the progeny of the injected cells do not participate in the secondary axis formation. In normal development, Siamois mRNA is first detected shortly after the midblastula transition, which is earlier than mRNAs for goosecoid or Xbrachyury, and is present most abundantly in the dorsal endoderm of early gastrulae. The activation of this gene can be obtained cell autonomously in dispersed embryo cells. These results indicate that Siamois may play an important role in the formation of the Nieuwkoop center.