Effect of supplementation of leukemia inhibitory factor and epidermal growth factor on murine embryonic development in vitro, implantation, and outcome of offspring
Effect of supplementation of leukemia inhibitory factor and epidermal growth factor on murine embryonic development in vitro, implantation, and outcome of offspring
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DOI：
10.1016/s0015-0282(03)00772-6
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发表时间：
2003-09-01
影响因子：
6.7
通讯作者：
Bongso, A
中科院分区：
文献类型：
--
作者：
Cheung, LP;Leung, HY;Bongso, A
目的：评价在单一培养系统（IVF50）和顺序培养系统（IVF50- s2）中添加白血病抑制因子（LIF）或表皮生长因子（EGF）是否对胚胎发育和着床有额外的益处。设计：前瞻性对照动物研究。环境：大学研究实验室。实验对象：F1（CBA X C57BL）小鼠的双细胞胚胎。干预：胚胎在不同的生长因子处理或未处理的体外系统中随机培养。主要观察指标：囊胚形成和形态，第5天囊胚总细胞数，出生率，子代特征。结果：仅在IVF50培养基中观察到LIF或EGF对囊胚发育和形态的有益影响，而在连续的IVF50- s2培养基中没有观察到。此外，与单独使用IVF50培养基的囊胚相比，添加lif培养物产生的囊胚在移植后具有更高的总细胞数和更高的总出生率。未观察到对胎儿发育的显著影响，但在添加了liff的顺序培养物中接受治疗后出生的幼崽妊娠期延长，出生体重增加。结论：LIF和EGF对体外囊胚发育的有益作用主要出现在次优培养条件下（仅在简单培养基中），其作用在改进的体外系统（顺序培养基）中被掩盖。然而，在培养基中添加这些因子仍然是通过增加总细胞数来提高胚胎活力的一种有吸引力的方法。LIF在改善着床和提高出生率方面的有益作用有待进一步探讨。(C) 2003年，美国生殖医学学会。
Objective: To evaluate the supplementation of leukemia inhibitory factor (LIF) or epidermal growth factor (EGF) in a single culture system (IVF50) and a sequential culture system (IVF50-S2) to determine whether they had any additional benefit on embryo development and implantation.Design: Prospective controlled animal study.Setting: University research laboratory.Subject(s): Two-cell embryos from F1(CBA X C57BL) mice.Intervention(s): Embryos were randomly cultured in different growth factor-treated or untreated in vitro systems.Main Outcome Measure(s): Blastocyst formation and morphology, total cell numbers of day 5 blastocysts, birth rates, and characteristics of the offspring.Result(s): The beneficial effects of LIF or EGF on blastocyst development and morphology were observed only in IVF50 medium but not in sequential IVF50-S2 media. In addition, blastocysts generated from the LIF-supplemented cultures had higher total cell numbers and higher total birth rates after transfer compared to those from IVF50 medium alone. No significant effect on fetal development was observed but pups born after treatment in LIF-supplemented sequential cultures had prolonged gestation and increased birth weights.Conclusion(s): The beneficial effects of LIF and EGF on in vitro blastocyst development are mainly seen under suboptimal culture conditions (simple medium only) and their effects are masked in an improved in vitro system (sequential culture media). Supplementation of culture media with such factors, however, is still an attractive approach in enhancing embryo viability through increased total cell numbers. The beneficial effect of LIF in improving implantation and subsequent increased birth rates should be further explored. (C) 2003 by American Society for Reproductive Medicine.