Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs
Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs
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DOI：
10.1007/s007050050694
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发表时间：
2000-05
影响因子：
2.7
通讯作者：
S. Pappu;A. Bhat;H. Pappu;C. Deom;A. Culbreath
中科院分区：
文献类型：
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作者：
S. Pappu;A. Bhat;H. Pappu;C. Deom;A. Culbreath
对圆球病毒（布尼亚病毒科）S 和 M RNA 的基因间区域 (IGR) 的分析表明它们在长度和序列上均存在异质性。一般来说，M RNA 的 IGR 与其各自 S RNA 种类的 IGR 相比，长度较短。不同物种的 S RNA IGR 序列之间的同一性百分比为 42% 至 57%，而同一物种的分离株之间的同一性百分比为 79% 至 99%。同样，当比较 M RNA 的 IGR 时，相同拓扑病毒属的分离株之间的序列同一性（84% 至 98%）比不同的拓扑病毒属之间的序列同一性（46% 至 59%）更高。 S 和 M RNA 的 IGR 序列的核苷酸同一性百分比和最大似然树表明，它们在种间和种内水平上的序列差异与核衣壳基因的序列差异相似。这是对已知拓扑病毒 S 和 M RNA 的 IGR 的首次详细序列分析。
Analysis of the intergenic region (IGR) of S and M RNAs of tospoviruses (FamilyBunyaviridae) indicated their heterogeneity both in length and sequence. In general, IGRs of M RNA were shorter in length compared to the IGRs of their respective S RNA species. Percent identity among the S RNA IGR sequences of distinct tospovirus species varied from 42 to 57%, whereas it was 79 to 99% among isolates of the same species. Similarly, when IGRs of M RNAs were compared, there was higher sequence identity among isolates of the same tospovirus species (84 to 98%) than among distinct tospovirus species (46 to 59%). Percent nucleotide identities and maximum likelihood trees of IGR sequences of S and M RNAs indicated that their sequence divergence is similar to that of nucleocapsid gene at inter and intra-species levels. This is the first detailed sequence analysis of IGRs of S and M RNAs of known tospoviruses.