INFECTIVITY ASSAY AND NEUTRALIZATION TEST FOR EQUINE INFLUENZA-VIRUS IN MICROPLATE CELL-CULTURES
INFECTIVITY ASSAY AND NEUTRALIZATION TEST FOR EQUINE INFLUENZA-VIRUS IN MICROPLATE CELL-CULTURES
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DOI：
10.1016/0378-1135(81)90024-9
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发表时间：
1981-01-01
影响因子：
3.3
通讯作者：
MATUMOTO, M
中科院分区：
文献类型：
--
作者：
YAMAGISHI, H;NAGAMINE, T;MATUMOTO, M
对包括非洲绿猴肾Vero和Madin-Darby犬肾MDCK细胞在内的几种细胞系进行了对马流感病毒迈阿密和布拉格毒株的易感性测试，以找到适合研究这些病毒的细胞培养物。在这些细胞培养物中，病毒很容易繁殖并产生细胞病变效应。从猪胚肾中提取的ESK细胞对病毒最敏感。利用在微孔板上制备的ESK细胞培养物对病毒进行了传染性试验。该方法不仅易于常规使用，而且实际上与鸡蛋接种法一样敏感。该方法进一步适用于中和试验。
Several cell lines including African green monkey kidney Vero and Madin-Darby canine kidney MDCK cells were tested for susceptibility to the Miami and Prague strains of equine influenza virus to find cell cultures suited for study of these viruses. The viruses readily multiplied with cytopathic effects in these cell cultures. ESK cells derived from swine embryo kidney were the most susceptible to the viruses. An infectivity assay of the viruses was worked out using ESK cell cultures prepared in microplates. The method is not only simple enough for routine use but is practically as sensitive as the egg inoculation method. The method was further adapted to a neutralization test.