Generation of Sex-Reversed Female Clonal Mice via CRISPR-Cas9-Mediated Y Chromosome Deletion in Male Embryonic Stem Cells.
Generation of Sex-Reversed Female Clonal Mice via CRISPR-Cas9-Mediated Y Chromosome Deletion in Male Embryonic Stem Cells.
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通过 CRISPR-Cas9 介导的雄性胚胎干细胞 Y 染色体缺失产生性别逆转的雌性克隆小鼠。
DOI：
10.1089/crispr.2020.0074
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wen,Duancheng
中科院分区：
文献类型：
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作者：
Qin,Yiren;Wong,Bokey;Zhong,Liangwen;Geng,Fuqiang;Parada,LuisF;Wen,Duancheng
完全源自胚胎干（ES）细胞的小鼠可以通过四倍体互补产生。虽然该系统中通常使用 XY 雄性 ES 细胞系，但有时，单体 XO 雌性小鼠是通过自发 Y 染色体丢失产生的。在这里，我们描述了一种通过 CRISPR-Cas9 介导的 Y 染色体缺失获得单体 XO ES 细胞的有效方法，从而通过四倍体互补产生雌性克隆小鼠。单体 XO 雌性小鼠可存活并能够产生正常的雄性和雌性后代。直接产生两性克隆小鼠可以显着加速复杂转基因小鼠模型的产生。
Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice are produced through spontaneous Y chromosome loss. Here, we describe an efficient method to obtain monosomic XO ES cells by CRISPR-Cas9-mediated deletion of the Y chromosome, allowing generation of female clonal mice by tetraploid complementation. The monosomic XO female mice are viable and able to produce normal male and female offspring. Direct generation of clonal mice in both sexes can significantly accelerate the production of complex genetically modified mouse models.