Comparison of the localization of tetrodotoxin between wild pufferfish Takifugu rubripes juveniles and hatchery-reared juveniles with tetrodotoxin administration
Comparison of the localization of tetrodotoxin between wild pufferfish Takifugu rubripes juveniles and hatchery-reared juveniles with tetrodotoxin administration
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DOI：
10.1016/j.toxicon.2013.05.018
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发表时间：
2013-09-01
期刊：
影响因子：
2.8
通讯作者：
Sakakura, Yoshitaka
中科院分区：
文献类型：
--
作者：
Okita, Kogen;Takatani, Tomohiro;Sakakura, Yoshitaka
研究河豚毒素（TTX）在红鳍东方鲀幼鱼体内的积累规律。我们比较了TTX在野生幼鱼和孵化场饲养的幼鱼中的各种组织中的定位（使用抗TTX单克隆抗体的免疫组织化学技术施用）。结果表明，在体长为4.7- 9.4cm的野生幼鱼的肝组织、皮肤基底细胞、嗅细胞、嗅上皮、视神经和脑（视顶盖、小脑、延髓）中均观察到免疫阳性反应。经口给药后，在与野生幼鱼相同的组织和孵化幼鱼（BL，5.0-5.3 cm）肠上皮细胞层中检测到TTX。未注射TTX的孵化场饲养幼鱼组织中未观察到阳性反应。这些结果表明，口服TTX的无毒养殖的青少年是积累在相同的方式野生青少年。此外，我们的研究表明，河豚鱼在中枢神经系统中积累TTX。(C)2013爱思唯尔有限公司保留所有权利。
To reveal the accumulation profile of tetrodotoxin (TTX) in pufferfish Takifugu rubripes juveniles,. we compared the localization of TTX in various tissues among wild juveniles and hatchery-reared juveniles with or without TTX( administration using immunohistochemical technique with anti-TTX monoclonal antibody. Immuno-positive reaction was observed in hepatic tissue, basal cell of skin and olfactory, olfactory epithelium, optic nerve and brain (optic tectum, cerebellum, medulla oblongata) of wild juveniles (body length: BL, 4.7-9.4 cm). TTX was detected in the same tissues as wild juveniles and epithelial cell layer of intestine of hatchery-reared juveniles (BL, 5.0-5.3 cm) to which TTX was orally administrated. No positive reaction was observed from the tissues of hatchery-reared juveniles without TTX administration. These results suggest that orally administrated TTX to the non-toxic cultured juveniles is accumulated in the same manner of wild juveniles. In addition, our study revealed that pufferfish accumulates TTX in the central nervous system. (C) 2013 Elsevier Ltd. All rights reserved.