Isolation and Characterization of a Novel Betacoronavirus Subgroup A Coronavirus, Rabbit Coronavirus HKU14, from Domestic Rabbits
Isolation and Characterization of a Novel Betacoronavirus Subgroup A Coronavirus, Rabbit Coronavirus HKU14, from Domestic Rabbits
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DOI：
10.1128/jvi.06927-11
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发表时间：
2012-05-01
影响因子：
5.4
通讯作者：
Yuen, Kwok-Yung
中科院分区：
文献类型：
--
作者：
Lau, Susanna K. P.;Woo, Patrick C. Y.;Yuen, Kwok-Yung
我们描述了从家兔中分离和鉴定一种新型 Betacoronavirus A 亚组冠状病毒，即兔冠状病毒 HKU14 (RbCoV HKU14)。通过逆转录酶PCR（RT-PCR）在136份兔粪便样本中的11份（8.1%）中检测到病毒，病毒载量高达10（8）拷贝/ml。 RbCoV HKU14 能够在 HRT-18G 和 RK13 细胞中复制，并具有细胞病变效应。 Northern印迹证实了编码HE、S、NS5a、E、M和N蛋白的亚基因组mRNA的产生。亚基因组 mRNA 分析揭示了转录调控序列 5'-UCAAAC-3'。系统发育分析表明，RbCoV HKU14 在 Betacoronavirus A 亚组冠状病毒中形成了一个独特的分支，与 Betacoronavirus 1 物种关系最密切，但又相互独立。对保守复制酶结构域的比较表明，RbCoV HKU14 具有
We describe the isolation and characterization of a novel Betacoronavirus subgroup A coronavirus, rabbit coronavirus HKU14 (RbCoV HKU14), from domestic rabbits. The virus was detected in 11(8.1%) of 136 rabbit fecal samples by reverse transcriptase PCR (RT-PCR), with a viral load of up to 10(8) copies/ml. RbCoV HKU14 was able to replicate in HRT-18G and RK13 cells with cytopathic effects. Northern blotting confirmed the production of subgenomic mRNAs coding for the HE, S, NS5a, E, M, and N proteins. Subgenomic mRNA analysis revealed a transcription regulatory sequence, 5'-UCUAAAC-3'. Phylogenetic analysis showed that RbCoV HKU14 formed a distinct branch among Betacoronavirus subgroup A coronaviruses, being most closely related to but separate from the species Betacoronavirus 1. A comparison of the conserved replicase domains showed that RbCoV HKU14 possessed