An antibody to VacA of Helicobacter pylori in the CSF of patients with Miller-Fisher syndrome
An antibody to VacA of Helicobacter pylori in the CSF of patients with Miller-Fisher syndrome
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DOI：
10.1212/01.wnl.0000145705.82690.04
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发表时间：
2004-12-14
期刊：
影响因子：
9.9
通讯作者：
Hirayama, T
中科院分区：
文献类型：
--
作者：
Chiba, S;Sugiyama, T;Hirayama, T
作者检测了12例Miller-Fisher综合征（MFS）患者脑脊液中抗幽门螺杆菌天然空泡细胞毒素（VacA）的抗体。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离VacA蛋白，并进行Western blotting分析。12例MFS患者中有8例脑脊液中有针对VacA的特异性免疫球蛋白G抗体。VacA与一些膜离子转运蛋白之间存在序列同源性，这增加了A-VacA-Ab参与一些MFS患者朗维耶结离子通道的可能性。
The authors examined antibodies against native vacuolating cytotoxin (VacA) of Helicobacter pylori in CSF from 12 patients with Miller-Fisher syndrome (MFS). The VacA protein was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting analysis was carried out. Eight of 12 MFS patients had a specific immunoglobulin G antibody against VacA in the CSF. There is sequence homology between VacA and some membrane ion transport proteins, raising the possibility that A-VacA-Ab involves the ion channels in the node of Ranvier in some patients with MFS.