A Three-Enzyme Pathway with an Optimised Geometric Arrangement to Facilitate Substrate Transfer.
A Three-Enzyme Pathway with an Optimised Geometric Arrangement to Facilitate Substrate Transfer.
复制标题
具有优化几何排列以促进底物转移的三酶途径。
DOI：
10.1002/cbic.201600103
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yan,Hao
中科院分区：
文献类型：
--
作者：
Liu,Minghui;Fu,Jinglin;Qi,Xiaodong;Wootten,Shaun;Woodbury,NealW;Liu,Yan;Yan,Hao
级联反应驱动和调节多种代谢活动。酶之间底物运输的有效耦合对于整体途径活性非常重要，并且还控制驱动反应前进的中间分子的消耗。在这里，我们在一系列 DNA 纳米支架上组装了三酶途径，以研究它们的活性对空间排列的依赖性。与之前的研究不同，三酶途径的整体活性较少依赖于酶间距离，而更多地依赖于将它们排列在 10-30nm 相对较小范围内的几何图案。通路中间体检测表明，组装的酶系统通过有效的反应耦合快速耗尽中间体分子。
Cascade reactions drive and regulate a variety of metabolic activities. Efficient coupling of substrate transport between enzymes is important for overall pathway activity and also controls the depletion of intermediate molecules that drive the reaction forward. Here, we assembled a three‐enzyme pathway on a series of DNA nanoscaffolds to investigate the dependence of their activities on spatial arrangement. Unlike previous studies, the overall activity of the three‐enzyme pathway relied less on inter‐enzyme distance and more on the geometric patterns that arranged them within a relatively small range of 10–30 nm. Pathway intermediate detection demonstrated that the assembled enzyme systems quickly depleted the intermediate molecules through efficient reaction coupling.