Interstitial cells of cajal in dysmotility in intestinal ischemia and reperfusion injury in rats
Interstitial cells of cajal in dysmotility in intestinal ischemia and reperfusion injury in rats
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DOI：
10.1016/j.jss.2006.04.022
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Kitajima, Masaki
中科院分区：
文献类型：
--
作者：
Shimojima, Naoki;Nakaki, Toshio;Kitajima, Masaki
背景。肠缺血再灌注（I/R）损伤是小肠移植中必然发生的情况。 I/R 可能会损害正常的胃肠蠕动。卡哈尔间质细胞 (ICC) 被称为胃肠道中的起搏细胞。本研究的目的是评估 ICC 在 I/R 损伤大鼠模型胃肠运动中的作用。材料和方法。通过闭塞肠系膜血管，然后再灌注，使 Wistar 大鼠遭受 30 或 80 分钟的肠缺血。在12小时或4天时切除小肠段。通过器官浴技术评估自发机械活动。肌间神经丛水平的 c-Kit 和 PGP9.5 的免疫阳性分别作为 ICC 和肠神经的标记物进行评估。结果。在缺血 80 分钟再灌注 12 小时的肠段中，与缺血 30 分钟再灌注 12 小时的肠段相比，肌肉收缩频率减少 25%（P < 0.05），而收缩幅度没有显着差异。这一变化与 c-Kit 免疫阳性率下降 70% (P < 0.01) 相关。肠道运动模式和 c-Kit 阳性细胞分布的这些变化均在 80 分钟缺血和 4 天再灌注后恢复。相比之下，PGP9.5的免疫阳性在任何I/R损伤组中均不受影响。结论。 ICC 的短暂功能变化是由长期 I/R 损伤引起的，但在 4 天后恢复，表明 ICC 在 I/R 损伤中破坏和恢复正常胃肠运动方面发挥着核心作用。
Background. Intestinal ischemia and reperfusion (I/R) injury is an obligatory occurrence in small bowel transplantation. I/R may impair the normal gastrointestinal motility. Interstitial cells of Cajal (ICC) are known as pacemaker cells in the gastrointestinal tract. The aim of this study was to assess the role of ICC in the gastrointestinal motility in a rat model of I/R injury.Materials and methods. Wistar rats were subjected to 30- or 80-min intestinal ischemia by occluding the mesenteric vessels followed by reperfusion. Small intestinal segments were resected at 12 h or 4 days. The spontaneous mechanical activity was evaluated by organ bath technique. Immunopositivity of c-Kit and PGP9.5 at the level of the myenteric plexus was evaluated as markers of ICC and enteric nerves, respectively.Results. In the bowel segment with 80-min ischemia followed by 12-h reperfusion, muscles showed a 25% reduction (P < 0.05) in the frequency of contractions compared to that with 30-min ischemia followed by 12-h reperfusion, whereas amplitude of contractions was not significantly different. This change was associated with a 70% decrease (P < 0.01) of c-Kit immunopositivity. These changes of intestinal motility pattern and distribution of c-Kit-positive cells were both recovered from 80-min ischemia followed by 4 days reperfusion. In contrast, the immunopositivity of PGP9.5 was not affected in any I/R injury group.Conclusions. Transient functional changes in ICC were induced by prolonged I/R injury but they recovered after 4 days, suggesting a central role of ICC in both disrupting and restoring the normal gastrointestinal motility in I/R injury.