Use of arbitrarily primed polymerase chain reaction analysis to type disease and carrier strains of Neisseria meningitidis isolated during a university outbreak.
Use of arbitrarily primed polymerase chain reaction analysis to type disease and carrier strains of Neisseria meningitidis isolated during a university outbreak.
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使用任意引发的聚合酶链反应分析来确定大学暴发期间分离出的脑膜炎奈瑟氏菌的疾病类型和携带菌株。
DOI：
10.1093/infdis/169.6.1384
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Berg,DE
中科院分区：
文献类型：
--
作者：
Woods,JP;Kersulyte,D;TolanJr,RW;Berg,CM;Berg,DE
采用随机引物聚合酶链反应（PCR）或随机扩增多态性DNA（RAPD）方法对来自美国两个地区的脑膜炎奈瑟氏菌疾病和携带者分离株进行分型。该技术使用低严格性PCR和单个任意选择的引物产生扩增的DNA片段的菌株特异性阵列。使用4种引物中的每一种都无法区分来自康涅狄格大学爆发的3种疾病分离株和11种携带者分离株中的7种。相比之下，使用相同的4个引物将22个其他分离株（剩余的4个携带者分离株加上来自康涅狄格州、伊利诺伊州和密苏里州的18个疾病和携带者分离株）分成18组。这一结果支持了这样的观点，即来自暴发的疾病分离株可能反映了一种菌株的散发性侵袭性进展，这种菌株也经常导致无症状的定植。我们的研究结果表明，RAPD测试提供了一个敏感和有效的手段，区分遗传不同的脑膜炎球菌菌株，他们应该促进临床，流行病学和人口遗传学研究这一重要的病原体。
Disease and carrier isolates ofNeisseria meningitidisfrom two regions of the United States were typed by the arbitrarily primed polymerase chain reaction (PCR), or random amplified polymorphic DNA (RAPD), method. This technique generates strain-specific arrays of amplified DNA fragments using low-stringency PCR with single, arbitrarily chosen primers. Each of 3 disease isolates and 7 of 11 carrier isolates from an outbreak at the University of Connecticut were indistinguishable using each of 4 primers. In contrast, 22 other isolates (the remaining 4 carrier isolates plus 18 disease and carrier isolates from Connecticut, Illinois, and Missouri) were divided into 18 sets using the same 4 primers. This outcome supports the view that disease isolates from an outbreak may reflect sporadic invasive progression by a strain that also frequently causes asymptomatic colonization. Our results show that RAPD tests provide a sensitive and efficient means of distinguishing genetically different meningococcal strains and that they should facilitate clinical, epidemiologic, and population genetic studies of this important pathogen.