Microorganisms associated particulate matter: A preliminary study
Microorganisms associated particulate matter: A preliminary study
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DOI：
10.1016/j.scitotenv.2014.02.006
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发表时间：
2014-05-01
影响因子：
9.8
通讯作者：
Elserougy, Safaa
中科院分区：
文献类型：
--
作者：
Alghamdi, Mansour A.;Shamy, Magdy;Elserougy, Safaa
本研究旨在确定 2012 年 12 月至 2013 年 4 月期间沙特阿拉伯吉达市区颗粒物 (PM) 的微生物质量。这是通过测定空气中与 PM10 和 PM2.5 相关的细菌、真菌和放线菌及其与气态污染物、O-3、SO2 和 NO2 以及气象因素（T 摄氏度、RH% 和 Ws）的关系来实现的。具有 PM10 和 PM2.5 选择性尺寸的大容量采样器以及玻璃纤维过滤器分别用于收集 PM10 和 PM2.5。将过滤器悬浮在磷酸盐缓冲液中，并将等分试样铺在胰蛋白酶大豆琼脂、麦芽提取物琼脂和淀粉酪蛋白琼脂培养基的表面上，分别用于细菌、真菌和放线菌相关 PM 的计数。 PM10和PM2.5平均浓度分别为159.9μg/m(3)和60μg/m(3)，PM2.5/PM10平均比值接近0.4。 O-3、SO2、NO2浓度平均分别为35.73μg/m(3)、38.1μg/m(3)、52.5μg/m(3)。真菌和放线菌相关的 PM 浓度低于细菌。 PM10 中的微生物负荷总和高于 PM2.5，但存在显着相关性 (r = 0.57，P
This study aims to determine the microbiological quality of particulate matter (PM) in an urban area in Jeddah, Saudi Arabia, during December 2012 to April 2013. This was achieved by the determination of airborne bacteria, fungi, and actinobacteria associated PM10 and PM2.5, as well as their relationships with gaseous pollutants, O-3, SO2 and NO2, and meteorological factors (T degrees C, RH% and Ws). High volume samplers with PM10 and PM2.5 selective sizes, and glass fiber filters were used to collect PM10 and PM2.5, respectively. The filters were suspended in buffer phosphate and aliquots were spread plated onto the surfaces of trypticase soy agar, malt extract agar, and starch casein agar media for counting of bacteria, fungi and actinobacteria-associated PM, respectively. PM10 and PM2.5 concentrations averaged 159.9 mu g/m(3) and 60 mu g/m(3), respectively, with the ratio of PM2.5/PM10 averaged similar to 0.4. The concentrations of O-3, SO2 and NO2 averaged 35.73 mu g/m(3), 38.1 mu g/m(3) and 52.5 mu g/m(3), respectively. Fungi and actinobacteria associated PM were found in lower concentrations than bacteria. The sum of microbial loads was higher in PM10 than PM2.5, however a significant correlation (r = 0.57, P