LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation
LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation
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DOI：
10.1039/c3an01307c
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发表时间：
2014-01-01
期刊：
影响因子：
4.2
通讯作者：
Kerman, Kagan
中科院分区：
文献类型：
--
作者：
Cheng, Xin R.;Daaboul, George G.;Kerman, Kagan
Self-aggregation of amyloid-beta (A beta) plays an important role in the pathogenesis of Alzheimer's disease (AD). Small molecule inhibitors of Ab fibril formation reduce the A beta-mediated neurotocixity. In this report, the interaction of amyloid-beta (A beta) with well-described modulators, (-)epigallocatechin-3-gallate (EGCG) and Zn(II), was detected using a LED-based interferometric reflectance imaging sensor (LED-IRIS) in a high-throughput and real-time format. Nucleation-based fibril growth strategy was employed, as the "seeds" of A beta were prepared in the presence of EGCG and Zn(II). The seeds were then covalently immobilized on the chip surface. Using microfluidics, A beta oligomers were exposed onto the seeds resulting in the elongation of fibrils, which was detected as the increase in the spot height. Monitoring the changes on the chip surface enabled to detect the efficacy of modulators to inhibit or facilitate the growth of A beta fibrils. The proof-of-concept study reported here introduces a novel platform to facilitate the screening of small molecules towards the discovery of promising AD therapeutics.
