Cross-resistance to human cationic antimicrobial peptides and to polymyxins mediated by the plasmid-encoded MCR-1?
Cross-resistance to human cationic antimicrobial peptides and to polymyxins mediated by the plasmid-encoded MCR-1?
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DOI：
10.1016/j.cmi.2017.03.015
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发表时间：
2017-09-01
影响因子：
14.2
通讯作者：
Nordmann, P.
中科院分区：
文献类型：
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作者：
Dobias, J.;Poirel, L.;Nordmann, P.
目的：评价作为人体免疫系统正常组成部分的阳离子抗菌肽（CAMP）获得性耐药是否可能选择对多粘菌素等抗生素肽的共耐药，因为它们具有相同的作用机制。我们的目的是评估产生最近鉴定的质粒编码的多粘菌素抗性决定因子MCR-1的菌株，MCR-1是一种磷酸乙醇胺转移酶，可以修饰革兰氏阴性菌的脂多糖结构。方法：采用人camp （cathelicidin LL-37、a-防御素5 （HD5）和β -防御素3 (HDB3)）对产生mcr -1和耐粘菌素的大肠杆菌或肺炎克雷伯菌进行体外敏感性研究。结果：在大肠杆菌和肺炎克雷伯菌中均未观察到由MCR-1或染色体机制介导的camp和粘菌素交叉耐药。结论：至少在大肠杆菌中，MCR-1质粒介导的多粘菌素耐药性的传播不太可能阻碍人类camp的未来治疗发展。(C) 2017年欧洲临床微生物学与传染病学会。Elsevier Ltd.出版。版权所有。
Objectives: To evaluate whether acquired resistance to cationic antimicrobial peptide (CAMP) group molecules, being normal components of the human immune system, may select co-resistance to antibiotic peptides such as polymyxins, considering they share the same mechanism of action. We aimed to evaluate strains producing the recently identified plasmid-encoded polymyxin resistance determinant MCR-1, which is a phosphoethanolamine transferase that modifies the lipopolysaccharide structure of Gram-negative bacteria.Methods: In vitro susceptibility studies were performed using human CAMPs, namely cathelicidin LL-37, a-defensin 5 (HD5), and beta-defensin 3 (HDB3), towards MCR-1-producing and colistin-resistant Escherichia coli or Klebsiella pneumoniae.Results: Cross-resistance to CAMPs and colistin mediated by MCR-1 or chromosomal mechanisms was neither observed in E. coli nor in K. pneumoniae.Conclusion: Future therapeutic development of human CAMPs is not likely to be impeded by the spread of MCR-1 plasmid-mediated resistance to polymyxins, at least in E. coli. (C) 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.