Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface.
Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface.
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原代肝细胞在聚对苯二甲酸乙二醇酯表面的粘附接触动力学。
DOI：
10.1016/j.biomaterials.2004.03.041
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Chan,Vincent
中科院分区：
文献类型：
--
作者：
Tan,WeeJin;Teo,GilbertP;Liao,Kin;Leong,KamW;Mao,Hai-Quan;Chan,Vincent
生物人工肝辅助装置的设计需要原代肝细胞在高分子生物材料上的有效附着。更好地理解这种细胞表面相互作用将有助于生物材料的最佳选择。在这项研究中，原代肝细胞的粘附接触动力学的聚对苯二甲酸乙二醇酯（PET）表面接枝聚（丙烯酸）（PAA）和包被胶原蛋白探测与共聚焦反射干涉相差显微镜（C-RICM）结合相差显微镜。当丙烯酸浓度从0增加到12 nmole/cm ~ 2时，PET表面的均方根粗糙度和胶原蛋白的吸附量均增加。C-RICM证明，尽管二维细胞扩散不显著，但在普通PET和PAA接枝PET（均具有胶原蛋白涂层）上接种后，肝细胞形成紧密粘附接触。在细胞接种后2小时，肝细胞在12 nmole/cm ~ 2 PAA接枝PET（有胶原涂层）上的标准化接触面积和粘附能分别比胶原涂层的普通PET高27%和114%。有趣的是，PAA接枝PET与胶原涂层上的肝细胞粘附片的生长动力学最佳拟合为R t0.5，并且与胶原涂层的普通PET上的肝细胞粘附片的生长动力学显著不同，其最佳拟合为R t0.25。总体而言，本研究证明了通过控制生物材料表面特性来调节粘附肝细胞的生物物理反应。
The design of bioartificial liver assist device requires an effective attachment of primary hepatocytes on polymeric biomaterials. A better understanding of this cell-surface interaction would aid the optimal choice of biomaterials. In this study, the adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) (PET) surface with grafted poly(acrylic acid) (PAA) and coated collagen is probed with confocal reflectance interference contrast microscopy (C-RICM) in conjunction with phase contrast microscopy. An increase of acrylic acid density from 0 to 12nmole/cm2raises both the root-mean-square surface roughness and amount of adsorbed collagen of PET surface. C-RICM demonstrates that hepatocytes form tight adhesion contacts upon seeding on both plain PET and PAA-grafted PET (both with collagen coating) despite the insignificant two-dimensional cell spreading. At two hours after cell seeding, the normalized contact area and adhesion energy of hepatocytes on 12nmole/cm2PAA-grafted-PET (with collagen coating) is 27% and 114% higher, respectively, than that on collagen coated plain PET. Interestingly, the growth kinetics of adhesion patch for hepatocyte on PAA-grafted PET with collagen coating is best fitted by R∝t0.5and is significantly different from that on collagen coated plain PET, which is best fitted by R∝t0.25. Overall, this study demonstrates the modulation of biophysical response of adherent hepatocytes through the control of the biomaterial surface properties.