Advanced beamline automation for biological crystallography experiments.
Advanced beamline automation for biological crystallography experiments.
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用于生物晶体学实验的先进光束线自动化。
DOI：
10.1107/s0907444906014120
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Earnest,Thomas
中科院分区：
文献类型：
--
作者：
Cork,Carl;O'Neill,James;Taylor,John;Earnest,Thomas
劳伦斯伯克利国家实验室开发了一种自动晶体安装/对准系统，并已安装在先进光源（ALS）的三个蛋白质晶体学实验站上；目前它正在 CHESS、NSLS 和 APS 的同步加速器晶体学光束线中实施。使用自动安装系统的好处包括（i）优化同步加速器光束时间的使用，（ii）促进先进的数据收集技术，（iii）收集更高质量的数据，（iv）降低晶体风险以及（v）探索实验方案的系统研究。下一代自动贴片机的开发正在进行中，该机在稳健性、自动对准和样品跟踪方面进行了改进，并开发了端到端数据流流程以允许远程数据收集和监控。
An automated crystal-mounting/alignment system has been developed at Lawrence Berkeley National Laboratory and has been installed on three of the protein-crystallography experimental stations at the Advanced Light Source (ALS); it is currently being implemented at synchrotron crystallography beamlines at CHESS, NSLS and the APS. The benefits to using an automounter system include (i) optimization of the use of synchrotron beam time, (ii) facilitation of advanced data-collection techniques, (iii) collection of higher quality data, (iv) reduction of the risk to crystals and (v) exploration of systematic studies of experimental protocols. Developments on the next-generation automounter with improvements in robustness, automated alignment and sample tracking are under way, with an end-to-end data-flow process being developed to allow remote data collection and monitoring.